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CD20 / MS4A1 (MS4A1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439116

MBS439116 is an antibody targeting MS4A1. Also known as CD20 / MS4A1 (B-Cell Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD20 / MS4A1, Monoclonal Antibody
Full Product Name
CD20 / MS4A1 (B-Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-MS4A1 antibody
Product Synonym Names
APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
IGEL/773
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
33-37kDa
OMIM
112210
3D Structure
ModBase 3D Structure for P11836
Cellular Localization
Predominantly Cell Surface with some cytoplasmic
Immunogen
Recombinant human MS4A1 protein
Hu-Chromosome Location
11q12.2
Positive Control
Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.
Applications Tested/Suitable for anti-MS4A1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-MS4A1 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-MS4A1 antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Monoclonal Antibody (IGEL/773)
Mouse Anti-MS4A1 Monoclonal Antibody – Cat# MBS439116

Immunohistochemistry (IHC) of anti-MS4A1 antibody

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Monoclonal Antibody (IGEL/773)
Mouse Anti-MS4A1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439116

Product References and Citations for anti-MS4A1 antibody

Tedder, T.F., et al. 1994. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol. Today 15: 450-454

Other Notes

Small volumes of anti-MS4A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MS4A1. It may not necessarily be applicable to this product.

NCBI GI #
23110987
NCBI GeneID
931
NCBI Accession #
NP_068769.2
UniProt Primary Accession #
P11836
UniProt Secondary Accession #
P08984; Q13963; A6NMS4; B4DT24
UniProt Related Accession #
P11836
GenBank Nucleotide #
NM_021950.3
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane spanning 4-domains A1
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Synonym Protein Names
B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20
Protein Family
B-lymphocyte antigen
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_HUMAN
NCBI Protein Information
B-lymphocyte antigen CD20

NCBI Summary for MS4A1

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for MS4A1

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Cell surface; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space

Molecular Function: protein binding

Disease: Immunodeficiency, Common Variable, 5
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Research Articles on MS4A1

1. Case Report: aberrant CD20 expression by skin-infiltrating T cells using multispectral imaging.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MS4A1 antibody

 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-MS4A1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >3 publications with MS4A1 and Adenocarcinoma
 Carcinoma Antibodies  >2 publications with MS4A1 and Carcinoma
 Atherosclerosis Antibodies  >2 publications with MS4A1 and Atherosclerosis
 Inflammation Antibodies  >2 publications with MS4A1 and Inflammation
 Carcinoma, Squamous Cell Antibodies  >1 publications with MS4A1 and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >1 publications with MS4A1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >1 publications with MS4A1 and Breast Neoplasms
 Lung Diseases Antibodies  >1 publications with MS4A1 and Lung Diseases
 Heart Diseases Antibodies  >1 publications with MS4A1 and Heart Diseases
 Lung Neoplasms Antibodies  >1 publications with MS4A1 and Lung Neoplasms

Organs/Tissues associated with anti-MS4A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15 publications with MS4A1 and Blood
 Kidney Antibodies  >3 publications with MS4A1 and Kidney
 Lung Antibodies  >3 publications with MS4A1 and Lung
 Vascular Antibodies  >1 publications with MS4A1 and Vascular
 Lymph Antibodies  >1 publications with MS4A1 and Lymph
 Pancreas Antibodies  >1 publications with MS4A1 and Pancreas
 Prostate Antibodies  >1 publications with MS4A1 and Prostate
 Connective Tissue Antibodies  >1 publications with MS4A1 and Connective Tissue
 Pharynx Antibodies  >1 publications with MS4A1 and Pharynx
 Tonsil Antibodies  >1 publications with MS4A1 and Tonsil
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