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Blood Group Antigen Lewis B (FUT3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439118

MBS439118 is an antibody targeting FUT3. Also known as Blood Group Antigen Lewis B Mouse Monoclonal Antibody. Reactive in Human, Guinea Pig. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Blood Group Antigen Lewis B (FUT3), Monoclonal Antibody
Full Product Name
Blood Group Antigen Lewis B Mouse Monoclonal Antibody
Product Gene Name
anti-FUT3 antibody
Product Synonym Names
Blood group Lewis alpha-4-fucosyltransferase; Fucosyltransferase 3; Fucosyltransferase III; FucT-III; FUT3; Galactoside 3(4)-L-fucosyltransferase; gastric mucin; leB; lewis antigen system; lewis B blood group antigen; Lewis FT; major airway glycoprotein
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM194
Host
Mouse
Species Reactivity
Human, Guinea Pig
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.
Preparation and Storage
Antibody with azide: store at 2 to 8°C. Non hazardous.
Molecular Weight
45kDa
OMIM
111100
3D Structure
ModBase 3D Structure for P21217
Cellular Localization
Goli apparatus, Golgi stock membrane
Immunogen
Mucin isolated from a human ovarian cyst fluid
Positive Control
Colon
Research Area
Cardiovascular
Applications Tested/Suitable for anti-FUT3 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-FUT3 antibody
Immunofluorescence:
(1-3ug/ml)

Immunohistochemistry (Formalin-fixed):
(1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-FUT3 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen Lewis B Mouse Monoclonal Antibody (SPM194).
Mouse Anti-FUT3 Monoclonal Antibody – Cat# MBS439118

SDS-PAGE of anti-FUT3 antibody

SDS-PAGE Analysis of Purified 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3 Mouse Monoclonal Antibody (2-25LE; same as LWB01). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-FUT3 Monoclonal Antibody, SDS-PAGE – Cat# MBS439118

Product References and Citations for anti-FUT3 antibody

Richman, P.I. et al. 1987. Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization. Int. J. Cancer. 39: 317-328. Rouger, P.h., et al, eds. 1987. Proceedings of the first international work- shop on monoclonal antibodies against human red blood cells and related antigens (Paris, 1987). Blood Transfusion Immunohaematology 30: 353- 720. Bara, J. et al. 1988. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem. J. 254: 185-193. Torrado J, Correa P, Ruiz B, Bernardi P, Zavala D, Bara J. Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30. Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara. Alteration of sialyl Lewis epitope expression in pterygium. J.Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631.

Other Notes

Small volumes of anti-FUT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FUT3. It may not necessarily be applicable to this product.

NCBI GI #
4503809
NCBI GeneID
2525
NCBI Accession #
NP_000140.1
UniProt Primary Accession #
P21217
UniProt Secondary Accession #
Q32NE7; Q99448; Q99449; B5U7U9; B5U7V0
UniProt Related Accession #
P21217
GenBank Nucleotide #
NM_000149.3
NCBI Official Full Name
galactoside 3(4)-L-fucosyltransferase
NCBI Official Synonym Full Names
fucosyltransferase 3 (Lewis blood group)
NCBI Official Symbol
FUT3
NCBI Official Synonym Symbols
LE; Les; FT3B; CD174; FucT-III
UniProt Protein Name
Galactoside 3(4)-L-fucosyltransferase
UniProt Synonym Protein Names
Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-III
Protein Family
Fucosyltransferase
UniProt Gene Name
FUT3
UniProt Synonym Gene Names
FT3B; LE; Lewis FT; FucT-III
UniProt Entry Name
FUT3_HUMAN
NCBI Protein Information
galactoside 3(4)-L-fucosyltransferase

NCBI Summary for FUT3

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for FUT3

FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.65; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; membrane

Molecular Function: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity

Biological Process: biopolymer glycosylation; cell-cell recognition; oligosaccharide biosynthetic process
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Research Articles on FUT3

1. Data suggest that fucosyltransferase 3 (FUT3) gene rs28362459 and rs3745635 single nucleotide polymorphisms (SNPs) may engender the increased risk of ileocolonic and ileal Crohn's disease (CD).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FUT3 antibody

 Reference Product  PubMed Publications
 FUT2 antibody  >45 publications with FUT3 and FUT2
 FUT1 antibody  >16 publications with FUT3 and FUT1
 ST3GAL6 antibody  >2 publications with FUT3 and ST3GAL6
 B3GALT5 antibody  >2 publications with FUT3 and B3GALT5
 GCNT2 antibody  >1 publications with FUT3 and GCNT2
 B3GNT3 antibody  >1 publications with FUT3 and B3GNT3
 ST3GAL3 antibody  >1 publications with FUT3 and ST3GAL3

Pathways associated with anti-FUT3 antibody

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 N-glycan Antennae Elongation In The Medial/trans-Golgi Pathway antibodies  N-glycan Antennae Elongation In The Medial/trans-Golgi Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Reactions Specific To The Complex N-glycan Synthesis Pathway antibodies  Reactions Specific To The Complex N-glycan Synthesis Pathway Diagram
 Transport To The Golgi And Subsequent Modification Pathway antibodies  Transport To The Golgi And Subsequent Modification Pathway Diagram

Diseases associated with anti-FUT3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with FUT3 and Inflammation
 Lung Diseases Antibodies  >5 publications with FUT3 and Lung Diseases
 Necrosis Antibodies  >5 publications with FUT3 and Necrosis
 Lung Neoplasms Antibodies  >4 publications with FUT3 and Lung Neoplasms
 Liver Diseases Antibodies  >4 publications with FUT3 and Liver Diseases
 Cardiovascular Diseases Antibodies  >4 publications with FUT3 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >3 publications with FUT3 and Atherosclerosis
 Heart Diseases Antibodies  >3 publications with FUT3 and Heart Diseases
 Neoplasms, Experimental Antibodies  >3 publications with FUT3 and Neoplasms, Experimental
 Fibrosis Antibodies  >3 publications with FUT3 and Fibrosis

Organs/Tissues associated with anti-FUT3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >9 publications with FUT3 and Lung
 Stomach Antibodies  >8 publications with FUT3 and Stomach
 Brain Antibodies  >4 publications with FUT3 and Brain
 Intestine Antibodies  >4 publications with FUT3 and Intestine
 Kidney Antibodies  >2 publications with FUT3 and Kidney
 Prostate Antibodies  >2 publications with FUT3 and Prostate
 Eye Antibodies  >1 publications with FUT3 and Eye
 Pancreas Antibodies  >1 publications with FUT3 and Pancreas
 Trachea Antibodies  >1 publications with FUT3 and Trachea
 Ascites Antibodies  >1 publications with FUT3 and Ascites
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