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CD34 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439119

MBS439119 is an antibody targeting CD34. Also known as CD34 (Hematopoietic Stem Cell & Endothelial Marker) Mouse Monoclonal Antibody. Reactive in Human, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD34, Monoclonal Antibody
Full Product Name
CD34 (Hematopoietic Stem Cell & Endothelial Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-CD34 antibody
Product Synonym Names
Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
HPCA1/1171
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and KAP'si s sarcoma, but shows low specificity.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
90-110kDa
OMIM
142230
3D Structure
ModBase 3D Structure for P28906
Cellular Localization
Cell Surface
Immunogen
Recombinant full-length human HPCA1 protein
Hu-Chromosome Location
1q32.2
Positive Control
KG-1 cells, Tonsil, or Angiosarcoma
Applications Tested/Suitable for anti-CD34 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-CD34 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-CD34 antibody

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)
Mouse Anti-CD34 Monoclonal Antibody – Cat# MBS439119

Immunohistochemistry (IHC) of anti-CD34 antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)
Mouse Anti-CD34 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439119

Flow Cytometry (FC/FACS) of anti-CD34 antibody

Flow Cytometry of KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).
Mouse Anti-CD34 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS439119

Product References and Citations for anti-CD34 antibody

Felshow DM et al. Blood 97:3768-3775 (2001). Sato T et al. Blood 94:2548-2554 (1999).

Other Notes

Small volumes of anti-CD34 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD34. It may not necessarily be applicable to this product.

NCBI GI #
68342038
NCBI GeneID
947
NCBI Accession #
NP_001020280.1
UniProt Primary Accession #
P28906
UniProt Secondary Accession #
Q15970; Q15971; Q5JTA3; Q5JTA4; Q9UJB1; A8K664
UniProt Related Accession #
P28906
GenBank Nucleotide #
NM_001025109.1
NCBI Official Full Name
hematopoietic progenitor cell antigen CD34 isoform a
NCBI Official Synonym Full Names
CD34 molecule
NCBI Official Symbol
CD34
UniProt Protein Name
Hematopoietic progenitor cell antigen CD34
UniProt Synonym Protein Names
CD_antigen: CD34
Protein Family
Hematopoietic progenitor cell antigen
UniProt Gene Name
CD34
UniProt Entry Name
CD34_HUMAN
NCBI Protein Information
hematopoietic progenitor cell antigen CD34

NCBI Summary for CD34

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
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UniProt Comments for CD34

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: apical plasma membrane; basal plasma membrane; cytoplasm; external side of plasma membrane; integral to plasma membrane; intercellular bridge; lysosome; perinuclear region of cytoplasm; plasma membrane

Molecular Function: carbohydrate binding; transcription factor binding

Biological Process: cell motility involved in cell locomotion; cell proliferation; cell-cell adhesion; cell-matrix adhesion; endothelial cell proliferation; glomerular filtration; hemopoiesis; leukocyte migration; negative regulation of blood coagulation; negative regulation of nitric oxide biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of angiogenesis; positive regulation of interleukin-10 production; positive regulation of odontogenesis; regulation of blood pressure; regulation of immune response; signal transduction; tissue homeostasis
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Research Articles on CD34

1. overexpression in glioma tissues associated closely with higher WHO grade (III + IV) [review]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD34 antibody

 Reference Product  PubMed Publications
 KIT antibody  >2181 publications with CD34 and KIT
 CD33 antibody  >1126 publications with CD34 and CD33
 CD19 antibody  >1061 publications with CD34 and CD19
 VEGFA antibody  >290 publications with CD34 and VEGFA
 PTPRC antibody  >104 publications with CD34 and PTPRC
 MYB antibody  >65 publications with CD34 and MYB
 CRKL antibody  >32 publications with CD34 and CRKL
 SELL antibody  >4 publications with CD34 and SELL
 DNTT antibody  >2 publications with CD34 and DNTT

Pathways associated with anti-CD34 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram

Diseases associated with anti-CD34 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >2466 publications with CD34 and Cardiovascular Diseases
 Necrosis Antibodies  >1250 publications with CD34 and Necrosis
 Adenocarcinoma Antibodies  >1003 publications with CD34 and Adenocarcinoma
 Anemia Antibodies  >902 publications with CD34 and Anemia
 Breast Neoplasms Antibodies  >888 publications with CD34 and Breast Neoplasms
 Disease Models, Animal Antibodies  >823 publications with CD34 and Disease Models, Animal
 Skin Neoplasms Antibodies  >820 publications with CD34 and Skin Neoplasms
 Inflammation Antibodies  >819 publications with CD34 and Inflammation
 Lung Diseases Antibodies  >761 publications with CD34 and Lung Diseases
 Liver Neoplasms Antibodies  >672 publications with CD34 and Liver Neoplasms

Organs/Tissues associated with anti-CD34 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17629 publications with CD34 and Blood
 Bone Antibodies  >10553 publications with CD34 and Bone
 Bone Marrow Antibodies  >9883 publications with CD34 and Bone Marrow
 Vascular Antibodies  >5844 publications with CD34 and Vascular
 Muscle Antibodies  >2698 publications with CD34 and Muscle
 Umbilical Cord Antibodies  >1837 publications with CD34 and Umbilical Cord
 Liver Antibodies  >1772 publications with CD34 and Liver
 Skin Antibodies  >1632 publications with CD34 and Skin
 Lung Antibodies  >1196 publications with CD34 and Lung
 Heart Antibodies  >1143 publications with CD34 and Heart
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