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Transglutaminase II (TGM2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439138

MBS439138 is an antibody targeting TGM2. Also known as Transglutaminase II (TGM2) Mouse Monoclonal Antibody. Reactive in Human, Monkey, Rabbit, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Transglutaminase II (TGM2), Monoclonal Antibody
Full Product Name
Transglutaminase II (TGM2) Mouse Monoclonal Antibody
Product Gene Name
anti-TGM2 antibody
Product Synonym Names
G alpha h; G[a]h; GNAH; Guanine nucleotide binding protein H polypeptide; Protein-glutamine gamma-glutamyltransferase 2; TG(C); TG2; TGase C; TGase H; TGase-2; TgaseII; TGC; TGM2; Tissue type transglutaminase; Transglutaminase 2 C polypeptide; Transglutaminase C; Transglutaminase H; Transglutaminase-2; tTG; tTGas
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
TGM2/419
Host
Mouse
Species Reactivity
Human, Monkey, Rabbit, Mouse, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a 77-85kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
77-85kDa
OMIM
190196
3D Structure
ModBase 3D Structure for P21980
Cellular Localization
Cytoplasmic and Cell Surface
Immunogen
Recombinant full-length human TGM2 protein
Hu-Chromosome Location
20q12
Positive Control
HUVEC Cells. Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin (e.g. intestine)
Applications Tested/Suitable for anti-TGM2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-TGM2 antibody
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-TGM2 antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Transglutaminase II Monoclonal Antibody (TGM2/419)
Mouse Anti-TGM2 Monoclonal Antibody – Cat# MBS439138

Immunohistochemistry (IHC) of anti-TGM2 antibody

Formalin-fixed, paraffin-embedded human Placenta stained with Transglutaminase II Monoclonal Antibody (TGM2/419)
Mouse Anti-TGM2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439138

Immunohistochemistry (IHC) of anti-TGM2 antibody

Formalin-fixed, paraffin-embedded human Uterus stained with Transglutaminase II Monoclonal Antibody (TGM2/419)
Mouse Anti-TGM2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439138

Immunohistochemistry (IHC) of anti-TGM2 antibody

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Transglutaminase II Monoclonal Antibody (TGM2/419)
Mouse Anti-TGM2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439138

Product References and Citations for anti-TGM2 antibody

Yamanishi, K., et al. 1991. Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture. Biochem. Biophys. Res. Commun. 175: 906-913.

Other Notes

Small volumes of anti-TGM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TGM2. It may not necessarily be applicable to this product.

NCBI GI #
1020158911
NCBI GeneID
7052
NCBI Accession #
NP_001310245.1
UniProt Primary Accession #
P21980
UniProt Secondary Accession #
Q16436; Q6B838; Q9BTN7; Q9UH35; E1P5V9
UniProt Related Accession #
P21980
GenBank Nucleotide #
NM_001323316.1
NCBI Official Full Name
protein-glutamine gamma-glutamyltransferase 2 isoform a
NCBI Official Synonym Full Names
transglutaminase 2
NCBI Official Symbol
TGM2
NCBI Official Synonym Symbols
TGC; TG(C)
UniProt Protein Name
Protein-glutamine gamma-glutamyltransferase 2
UniProt Synonym Protein Names
Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2
Protein Family
Protein-glutamine gamma-glutamyltransferase
UniProt Gene Name
TGM2
UniProt Synonym Gene Names
TG(C); TGC; TGase C; TGase H; TGase-2
UniProt Entry Name
TGM2_HUMAN
NCBI Protein Information
protein-glutamine gamma-glutamyltransferase 2

NCBI Summary for TGM2

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for TGM2

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: cytosol; focal adhesion; intrinsic to plasma membrane; mitochondrion

Molecular Function: protein binding; protein-glutamine gamma-glutamyltransferase activity

Biological Process: apoptotic cell clearance; elevation of mitochondrial calcium ion concentration; positive regulation of apoptosis; positive regulation of cell adhesion; reduction of endoplasmic reticulum calcium ion concentration
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Research Articles on TGM2

1. Results show no genetic association of the TGM2 gene with schizophrenia in the Chinese cohort.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TGM2 antibody

 Products by Pathway  Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Thromboxane A2 Receptor Signaling Pathway antibodies  Thromboxane A2 Receptor Signaling Pathway Diagram

Diseases associated with anti-TGM2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >14 publications with TGM2 and Inflammation
 Necrosis Antibodies  >7 publications with TGM2 and Necrosis
 Breast Neoplasms Antibodies  >7 publications with TGM2 and Breast Neoplasms
 Fibrosis Antibodies  >6 publications with TGM2 and Fibrosis
 Disease Models, Animal Antibodies  >6 publications with TGM2 and Disease Models, Animal
 Lung Neoplasms Antibodies  >6 publications with TGM2 and Lung Neoplasms
 Brain Neoplasms Antibodies  >5 publications with TGM2 and Brain Neoplasms
 Carcinoma, Hepatocellular Antibodies  >5 publications with TGM2 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >5 publications with TGM2 and Cardiovascular Diseases
 Kidney Diseases Antibodies  >5 publications with TGM2 and Kidney Diseases

Organs/Tissues associated with anti-TGM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >12 publications with TGM2 and Lung
 Blood Antibodies  >11 publications with TGM2 and Blood
 Liver Antibodies  >10 publications with TGM2 and Liver
 Brain Antibodies  >10 publications with TGM2 and Brain
 Eye Antibodies  >9 publications with TGM2 and Eye
 Bone Antibodies  >9 publications with TGM2 and Bone
 Connective Tissue Antibodies  >7 publications with TGM2 and Connective Tissue
 Vascular Antibodies  >7 publications with TGM2 and Vascular
 Skin Antibodies  >7 publications with TGM2 and Skin
 Muscle Antibodies  >6 publications with TGM2 and Muscle
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