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Tracp (Tartrate-Resistant Acid Phosphatase) (ACP5) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439147

MBS439147 is an antibody targeting ACP5. Also known as TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRAcP (Tartrate-Resistant Acid Phosphatase) (ACP5), Monoclonal Antibody
Full Product Name
TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-ACP5 antibody
Product Synonym Names
Acid phosphatase 5, tartrate resistant; ACP5; serum band 5 tartrate-resistant acid phosphatase; SPENCDI; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR-AP; TRACP5; TRAcP; TRAP; TrATPase; Type 5 acid phosphatase
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
SPM601
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher's Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
35kDa
OMIM
171640
3D Structure
ModBase 3D Structure for P13686
Cellular Localization
Cytoplasmic (Lysosomes)
Immunogen
Recombinant full-length human ACP5 protein
Hu-Chromosome Location
19p13.2
Positive Control
HepG2, 293T, K562 or RPMI-8226 Cells. Spleen from Hairy Cell Leukemia (HCL) patient.
Applications Tested/Suitable for anti-ACP5 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ACP5 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-ACP5 antibody

Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Monoclonal Antibody (SPM601)
Mouse Anti-ACP5 Monoclonal Antibody – Cat# MBS439147

Product References and Citations for anti-ACP5 antibody

Capeller, B., Caffier, H., Sutterlin, M.W. and Dietl, J. 2003. Evaluation of tartrate-resistant acid phosphatase (TRAP) 5b as serum marker of bone metastases in human breast cancer. Anticancer Res. 23: 1011-1015.

Other Notes

Small volumes of anti-ACP5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACP5. It may not necessarily be applicable to this product.

NCBI GI #
161377451
NCBI GeneID
54
NCBI Accession #
NP_001104504.1
UniProt Primary Accession #
P13686
UniProt Secondary Accession #
Q2TAB1; Q6IAS6; Q9UCJ5; Q9UCJ6; Q9UCJ7; A8K3V2
UniProt Related Accession #
P13686
GenBank Nucleotide #
NM_001111034.2
NCBI Official Full Name
tartrate-resistant acid phosphatase type 5
NCBI Official Synonym Full Names
acid phosphatase 5, tartrate resistant
NCBI Official Symbol
ACP5
NCBI Official Synonym Symbols
HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
UniProt Protein Name
Tartrate-resistant acid phosphatase type 5
UniProt Synonym Protein Names
Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase
Protein Family
Acyl carrier protein
UniProt Gene Name
ACP5
UniProt Synonym Gene Names
TR-AP; TrATPase
UniProt Entry Name
PPA5_HUMAN
NCBI Protein Information
tartrate-resistant acid phosphatase type 5

NCBI Summary for ACP5

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

UniProt Comments for ACP5

ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Protein type: Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis; EC 3.1.3.2; Phosphatase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytosol; integral to membrane

Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding

Biological Process: riboflavin metabolic process

Disease: Spondyloenchondrodysplasia With Immune Dysregulation
Show more

Research Articles on ACP5

1. Trap-5b is overexpressed in renal cell carcinoma patients with bone metastasis and bone resorption.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACP5 antibody

 Reference Product  PubMed Publications
 TNFSF11 antibody  >106 publications with ACP5 and TNFSF11
 CTSK antibody  >77 publications with ACP5 and CTSK
 TNFRSF11B antibody  >31 publications with ACP5 and TNFRSF11B
 SPP1 antibody  >12 publications with ACP5 and SPP1
 BGLAP antibody  >8 publications with ACP5 and BGLAP
 ENPP1 antibody  >1 publications with ACP5 and ENPP1

Pathways associated with anti-ACP5 antibody

 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 Osteoclast Signaling Pathway antibodies  Osteoclast Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 RANKL/RANK Signaling Pathway antibodies  RANKL/RANK Signaling Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram

Diseases associated with anti-ACP5 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with ACP5 and Inflammation
 Spondyloenchondrodysplasia Antibodies  >8 publications with ACP5 and Spondyloenchondrodysplasia
 Liver Diseases Antibodies  >4 publications with ACP5 and Liver Diseases
 Necrosis Antibodies  >4 publications with ACP5 and Necrosis
 Obesity Antibodies  >3 publications with ACP5 and Obesity
 Carcinoma, Hepatocellular Antibodies  >2 publications with ACP5 and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >2 publications with ACP5 and Lung Neoplasms
 Lung Diseases Antibodies  >2 publications with ACP5 and Lung Diseases
 Disease Models, Animal Antibodies  >2 publications with ACP5 and Disease Models, Animal
 Liver Neoplasms Antibodies  >2 publications with ACP5 and Liver Neoplasms

Organs/Tissues associated with anti-ACP5 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >54 publications with ACP5 and Bone
 Blood Antibodies  >9 publications with ACP5 and Blood
 Liver Antibodies  >8 publications with ACP5 and Liver
 Connective Tissue Antibodies  >8 publications with ACP5 and Connective Tissue
 Lung Antibodies  >3 publications with ACP5 and Lung
 Uterus Antibodies  >2 publications with ACP5 and Uterus
 Kidney Antibodies  >2 publications with ACP5 and Kidney
 Brain Antibodies  >2 publications with ACP5 and Brain
 Eye Antibodies  >1 publications with ACP5 and Eye
 Parathyroid Antibodies  >1 publications with ACP5 and Parathyroid
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