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CD22 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439152

MBS439152 is an antibody targeting CD22. Also known as CD22 / BL-CAM Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD22 / BL-CAM, Monoclonal Antibody
Full Product Name
CD22 / BL-CAM Mouse Monoclonal Antibody
Product Gene Name
anti-CD22 antibody
Product Synonym Names
B-lymphocyte cell adhesion molecule (BL-CAM); B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2); SIGLEC2; T-cell surface antigen Leu-14
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
MYG13
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Preparation and Storage
Antibody without azide - store at -20 to -80 degree C. Non-hazardous. No MSDS required.
Molecular Weight
130-140kDa
OMIM
107266
3D Structure
ModBase 3D Structure for P20273
Cellular Localization
Cell Surface
Immunogen
Human Raji cells
Hu-Chromosome Location
19q13.1
Positive Control
Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human tonsil and lymphocytes.
Applications Tested/Suitable for anti-CD22 antibody
Functional Studies, Flow Cytometry (FC), Immunofluorescence (IF)
Application Notes for anti-CD22 antibody
Flow Cytometry (1-2ug/million cells)
Optimal dilution for a specific application should be determined.

SDS-PAGE of anti-CD22 antibody

SDS-PAGE Analysis Purified CD22 Mouse Monoclonal Antibody (MYG13). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-CD22 Monoclonal Antibody – Cat# MBS439152

Flow Cytometry (FC) of anti-CD22 antibody

Flow Cytometric Analysis of Ramos cells using CD22 Mouse Monoclonal Antibody (MYG13) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Mouse Anti-CD22 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS439152

Product References and Citations for anti-CD22 antibody

Knapp, W et al. eds Leukocyte Typing IV, p190-192, Oxford University Press, Oxford, 1989 Schlossman SF et al. eds. Leukocyte Typing V, p523-503, Oxford University Press, Oxford, 1989. Tedder TF et al. CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol 15:481-504. Cyster JG and Goodnow CC. Tuning antigen receptor signaling by CD22: integrating cues from antigens and the microenvironment. Immunity 1997,6:509-517. Tuscano JM et al. Involvement of p72syk kinase, p53/56lyn kinase and phosphatidyl inositol-3 kinase in signal transduction via the human B lymphocyte antigen CD22. Eur J Immunol 1996, 26:1246-1252. Sato S et al. CD22 negatively and positively regulates signal transduction through the B lymphocyte antigen receptor. Semin Immunol 1998, 10:287-297

Other Notes

Small volumes of anti-CD22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD22. It may not necessarily be applicable to this product.

NCBI GI #
297374825
NCBI GeneID
933
NCBI Accession #
NP_001172028.1
UniProt Primary Accession #
P20273
UniProt Secondary Accession #
O95699; O95701; O95702; O95703; Q01665; Q32M46; Q92872; Q92873; Q9UQA6; F5GYU4; F5H7U3
UniProt Related Accession #
P20273
GenBank Nucleotide #
NM_001185099.1
NCBI Official Full Name
B-cell receptor CD22 isoform 2
NCBI Official Synonym Full Names
CD22 molecule
NCBI Official Symbol
CD22
NCBI Official Synonym Symbols
SIGLEC2; SIGLEC-2
UniProt Protein Name
B-cell receptor CD22
UniProt Synonym Protein Names
B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22
Protein Family
B-cell receptor
UniProt Gene Name
CD22
UniProt Synonym Gene Names
SIGLEC2; BL-CAM; Siglec-2
UniProt Entry Name
CD22_HUMAN
NCBI Protein Information
B-cell receptor CD22

UniProt Comments for CD22

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: plasma membrane

Molecular Function: protein binding
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Research Articles on CD22

1. results demonstrate that loss of high affinity CD22 ligands on GC B-cells occurs in both mice and humans through alternative mechanisms, unmasking CD22 relative to naive and memory B-cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD22 antibody

 Reference Product  PubMed Publications
 CD19 antibody  >660 publications with CD22 and CD19
 BCR antibody  >155 publications with CD22 and BCR
 CD79B antibody  >80 publications with CD22 and CD79B
 CD79A antibody  >73 publications with CD22 and CD79A
 LYN antibody  >48 publications with CD22 and LYN
 SYK antibody  >33 publications with CD22 and SYK
 GRB2 antibody  >10 publications with CD22 and GRB2
 PTPRC antibody  >4 publications with CD22 and PTPRC

Pathways associated with anti-CD22 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 CD22 Mediated BCR Regulation Pathway antibodies  CD22 Mediated BCR Regulation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD22 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >1008 publications with CD22 and Immune System Diseases
 Chromosome Aberrations Antibodies  >78 publications with CD22 and Chromosome Aberrations
 Inflammation Antibodies  >62 publications with CD22 and Inflammation
 Respiratory Tract Diseases Antibodies  >51 publications with CD22 and Respiratory Tract Diseases
 Necrosis Antibodies  >50 publications with CD22 and Necrosis
 Disease Models, Animal Antibodies  >49 publications with CD22 and Disease Models, Animal
 Lung Diseases Antibodies  >25 publications with CD22 and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >24 publications with CD22 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >23 publications with CD22 and Kidney Diseases
 Liver Diseases Antibodies  >20 publications with CD22 and Liver Diseases

Organs/Tissues associated with anti-CD22 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1098 publications with CD22 and Blood
 Bone Marrow Antibodies  >304 publications with CD22 and Bone Marrow
 Spleen Antibodies  >125 publications with CD22 and Spleen
 Lymph Node Antibodies  >115 publications with CD22 and Lymph Node
 Lymph Antibodies  >103 publications with CD22 and Lymph
 Skin Antibodies  >53 publications with CD22 and Skin
 Lung Antibodies  >35 publications with CD22 and Lung
 Kidney Antibodies  >33 publications with CD22 and Kidney
 Tonsil Antibodies  >31 publications with CD22 and Tonsil
 Thymus Antibodies  >30 publications with CD22 and Thymus
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