Mouse Anti-NUMA1 Monoclonal Antibody – Cat# MBS439176

Formalin-fixed, paraffin-embedded human Tonsil stained with NuMA Monoclonal Antibody (SPM300)

Nuclear Mitotic Apparatus Protein (NUMA1) Antibody – Mouse Monoclonal

Name: NUMA1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439176
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439176

MBS439176 is an antibody targeting NUMA1. Also known as Nuclear Mitotic Apparatus Protein (NuMA) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Nuclear Mitotic Apparatus Protein (NuMA) (NUMA1), Monoclonal Antibody

Full Product Name

Nuclear Mitotic Apparatus Protein (NuMA) Mouse Monoclonal Antibody

Product Gene Name

anti-NUMA1 antibody

Product Synonym Names

Nuclear Mitotic Apparatus Protein 1; NuMA protein; NUMA1; SP-H antigen; Structural nuclear protein;

Clonality

Monoclonal

Isotype

IgM, kappa

Clone Number

SPM300

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

228kDa

OMIM

164009

3D Structure

ModBase 3D Structure for Q14980

Cellular Localization

Nuclear

Immunogen

Colon carcinoma 174T cells

Hu-Chromosome Location

11q13

Positive Control

Exponentially growing any cultured human cells. Tonsil or lymph node.

Applications Tested/Suitable for anti-NUMA1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-NUMA1 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-NUMA1 antibody

Butschak G et al. New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. Acta Histochem 1995, 97(1):19-31 Compton DA and Cleveland DW. NuMA is required for the proper completion of mitosis. J Cell Biol 1993, 120(4):947-57 Price CM and Pettijohn DE. Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of purified NuMA protein. Exp Cell Res 1986, 166:295-311. Gueth-Hallonet C et al. Cleavage of the nuclear matrix protein NuMA during apoptosis. Exp Cell Res 1997, 233(1):21-24.

Other Notes

Small volumes of anti-NUMA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NUMA1. It may not necessarily be applicable to this product.

NCBI GI #

557440899

NCBI GeneID

4926

NCBI Accession #

NP_001273490.1

UniProt Primary Accession #

Q14980

UniProt Secondary Accession #

Q14981; Q9BTE9; H0YH75

UniProt Related Accession #

Q14980

GenBank Nucleotide #

NM_001286561.1

NCBI Official Full Name

nuclear mitotic apparatus protein 1 isoform 2

NCBI Official Synonym Full Names

nuclear mitotic apparatus protein 1

NCBI Official Symbol

NUMA1

NCBI Official Synonym Symbols

NUMA; NMP-22

UniProt Protein Name

Nuclear mitotic apparatus protein 1

UniProt Synonym Protein Names

Nuclear matrix protein-22; NMP-22; SP-H antigen

Protein Family

Nuclear mitotic apparatus protein

UniProt Gene Name

NUMA1

UniProt Synonym Gene Names

NMP-22

UniProt Entry Name

NUMA1_HUMAN

NCBI Protein Information

nuclear mitotic apparatus protein 1

NCBI Summary for NUMA1

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

UniProt Comments for NUMA1

NuMA-1: a coiled-coil nuclear protein that is required to establish and maintain mammalian spindle poles. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Two human splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cell cortex; centrosome; cytoplasm; cytosol; nucleoplasm; nucleus; spindle; spindle microtubule; spindle pole

Molecular Function: protein binding; structural molecule activity

Biological Process: nuclear organization and biogenesis

Disease: Acute Promyelocytic Leukemia

Research Articles on NUMA1

1. Suppressor APC domain containing 2 negatively regulates the localization of LGN at the cell cortex, likely by competing with NuMA for its binding

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NUMA1 antibody

Reference Product	PubMed Publications
CDK1 antibody	>5 publications with NUMA1 and CDK1
SMC3 antibody	>2 publications with NUMA1 and SMC3
EPB41L1 antibody	>2 publications with NUMA1 and EPB41L1
AURKA antibody	>2 publications with NUMA1 and AURKA
CCNB1 antibody	>1 publications with NUMA1 and CCNB1
NEDD1 antibody	>1 publications with NUMA1 and NEDD1
RAE1 antibody	>1 publications with NUMA1 and RAE1

Pathways associated with anti-NUMA1 antibody

Products by Pathway	Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Centrosome Maturation Pathway antibodies	Centrosome Maturation Pathway Diagram
G2/M Transition Pathway antibodies	G2/M Transition Pathway Diagram
Mitotic G2-G2/M Phases Pathway antibodies	Mitotic G2-G2/M Phases Pathway Diagram
Recruitment Of NuMA To Mitotic Centrosomes Pathway antibodies	Recruitment Of NuMA To Mitotic Centrosomes Pathway Diagram
Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway antibodies	Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway Diagram

Diseases associated with anti-NUMA1 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>11 publications with NUMA1 and Breast Neoplasms
Nervous System Diseases Antibodies	>7 publications with NUMA1 and Nervous System Diseases
Cardiovascular Diseases Antibodies	>6 publications with NUMA1 and Cardiovascular Diseases
Prostatic Diseases Antibodies	>4 publications with NUMA1 and Prostatic Diseases
Adenocarcinoma Antibodies	>4 publications with NUMA1 and Adenocarcinoma
Lung Diseases Antibodies	>3 publications with NUMA1 and Lung Diseases
Lung Neoplasms Antibodies	>3 publications with NUMA1 and Lung Neoplasms
Ovarian Diseases Antibodies	>2 publications with NUMA1 and Ovarian Diseases
Ovarian Neoplasms Antibodies	>2 publications with NUMA1 and Ovarian Neoplasms
Heart Diseases Antibodies	>1 publications with NUMA1 and Heart Diseases

Organs/Tissues associated with anti-NUMA1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>20 publications with NUMA1 and Blood
Nerve Antibodies	>12 publications with NUMA1 and Nerve
Brain Antibodies	>10 publications with NUMA1 and Brain
Bone Antibodies	>8 publications with NUMA1 and Bone
Lung Antibodies	>7 publications with NUMA1 and Lung
Skin Antibodies	>6 publications with NUMA1 and Skin
Connective Tissue Antibodies	>6 publications with NUMA1 and Connective Tissue
Bone Marrow Antibodies	>6 publications with NUMA1 and Bone Marrow
Kidney Antibodies	>6 publications with NUMA1 and Kidney
Muscle Antibodies	>4 publications with NUMA1 and Muscle