Mouse Anti-TNC Monoclonal Antibody – Cat# MBS439248

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Tenascin C Monoclonal Antibody (SPM319) at 4ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH 9.0; ABC detection system with DAB Chromogen. Note staining of connective tissue.

Tenascin C (TNC) Antibody – Mouse Monoclonal

Name: TNC Antibody; Monoclonal; Mouse; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439248
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439248

MBS439248 is an antibody targeting TNC. Also known as Tenascin C (Stromal Marker For Epithelial Malignancy) Mouse Monoclonal Antibody. Reactive in Human.Does not react with Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tenascin C (TNC), Monoclonal Antibody

Full Product Name

Tenascin C (Stromal Marker For Epithelial Malignancy) Mouse Monoclonal Antibody

Product Gene Name

anti-TNC antibody

Product Synonym Names

Cytotactin; Glioma-associated-extracellular matrix antigen; GMEM; GP 150-225; Hexabrachion (HXB); JI; Myotendinous antigen; Neuronectin; TNC

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

SPM319

Host

Mouse

Species Reactivity

Human.
Does not react with Rat

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

210kDa; 300kDa

OMIM

187380

3D Structure

ModBase 3D Structure for P24821

Cellular Localization

Connective tissue matrix

Immunogen

Human breast carcinoma

Hu-Chromosome Location

9q33

Positive Control

Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell, and lung carcinomas. Staining of normal fibroblasts serves as internal positive control

Applications Tested/Suitable for anti-TNC antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-TNC antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-TNC antibody

Verstraeten AA, et. al. British Journal of Dermatology, 1992, 127(6):571-4.

Other Notes

Small volumes of anti-TNC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNC. It may not necessarily be applicable to this product.

NCBI GI #

153946395

NCBI GeneID

3371

NCBI Accession #

NP_002151.2

UniProt Primary Accession #

P24821

UniProt Secondary Accession #

Q14583; Q15567; Q5T7S3; C9IYT7; C9J575; C9J6D9; C9J848

UniProt Related Accession #

P24821

GenBank Nucleotide #

NM_002160.3

NCBI Official Full Name

tenascin

NCBI Official Synonym Full Names

tenascin C

NCBI Official Symbol

TNC

NCBI Official Synonym Symbols

GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225

UniProt Protein Name

Tenascin

UniProt Synonym Protein Names

Cytotactin; GMEM; GP 150-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; JI; Myotendinous antigen; Neuronectin; Tenascin-C; TN-C

Protein Family

Tenascin

UniProt Gene Name

TNC

UniProt Synonym Gene Names

HXB; TN; TN-C

UniProt Entry Name

TENA_HUMAN

NCBI Protein Information

tenascin

NCBI Summary for TNC

This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]

UniProt Comments for TNC

TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: extracellular matrix; extracellular region; extracellular space; focal adhesion; membrane

Molecular Function: syndecan binding

Biological Process: extracellular matrix organization and biogenesis; osteoblast differentiation; response to wounding

Disease: Deafness, Autosomal Dominant 56

Research Articles on TNC

1. Serum TNC levels are significantly raised and correlate with various clinical and laboratory variables of disease activity in children with enthesitis-related arthritis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNC antibody

Reference Product	PubMed Publications
EGFR antibody	>14 publications with TNC and EGFR
RHOA antibody	>9 publications with TNC and RHOA
MMP3 antibody	>9 publications with TNC and MMP3
FGF2 antibody	>7 publications with TNC and FGF2
PAX6 antibody	>2 publications with TNC and PAX6
NPSR1 antibody	>1 publications with TNC and NPSR1
CNTN1 antibody	>1 publications with TNC and CNTN1
PTK2 antibody	>1 publications with TNC and PTK2
SDC4 antibody	>1 publications with TNC and SDC4

Pathways associated with anti-TNC antibody

Products by Pathway	Pathway Diagram
ECM Proteoglycans Pathway antibodies	ECM Proteoglycans Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-TNC antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>111 publications with TNC and Cardiovascular Diseases
Nervous System Diseases Antibodies	>99 publications with TNC and Nervous System Diseases
Heart Diseases Antibodies	>79 publications with TNC and Heart Diseases
Disease Models, Animal Antibodies	>59 publications with TNC and Disease Models, Animal
Brain Diseases Antibodies	>57 publications with TNC and Brain Diseases
Inflammation Antibodies	>53 publications with TNC and Inflammation
Lung Diseases Antibodies	>30 publications with TNC and Lung Diseases
Necrosis Antibodies	>27 publications with TNC and Necrosis
Breast Neoplasms Antibodies	>24 publications with TNC and Breast Neoplasms
Fibrosis Antibodies	>23 publications with TNC and Fibrosis

Organs/Tissues associated with anti-TNC antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>624 publications with TNC and Muscle
Heart Antibodies	>248 publications with TNC and Heart
Brain Antibodies	>140 publications with TNC and Brain
Bone Antibodies	>134 publications with TNC and Bone
Bone Marrow Antibodies	>102 publications with TNC and Bone Marrow
Embryonic Tissue Antibodies	>51 publications with TNC and Embryonic Tissue
Lung Antibodies	>51 publications with TNC and Lung
Skin Antibodies	>26 publications with TNC and Skin
Liver Antibodies	>24 publications with TNC and Liver
Connective Tissue Antibodies	>24 publications with TNC and Connective Tissue