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Fascin-1 (FSCN1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439383

MBS439383 is an antibody targeting FSCN1. Also known as Fascin-1 (Reed-Sternberg Cell Marker) Mouse Monoclonal Antibody. Reactive in Human, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Fascin-1 (FSCN1), Monoclonal Antibody
Full Product Name
Fascin-1 (Reed-Sternberg Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-FSCN1 antibody
Product Synonym Names
55kDa actin-bundling protein; FAN1; Fascin homolog 1; Fascin1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); SNL
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
FSCN1/418
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
55kDa
OMIM
602689
3D Structure
ModBase 3D Structure for Q16658
Cellular Localization
Cytoplasmic
Immunogen
Full length recombinant human FSCN1 protein
Hu-Chromosome Location
7p22.1
Positive Control
HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.
Applications Tested/Suitable for anti-FSCN1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-FSCN1 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-FSCN1 antibody

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Fascin-1 Monoclonal Antibody (FSCN1/418)
Mouse Anti-FSCN1 Monoclonal Antibody – Cat# MBS439383

Immunohistochemistry (IHC) of anti-FSCN1 antibody

Formalin-fixed, paraffin-embedded Rat Brain stained with Fascin-1 Monoclonal Antibody (FSCN1/418)
Mouse Anti-FSCN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439383

Product References and Citations for anti-FSCN1 antibody

Yamashiro-Matsumura S and Matsumura F. J Biol Chem 1985; 260(8): 5087. Yamashro-Matsumura S and Matsumura F. J Cell Biol 1986; 103:631. Duh F-M, et al. DNA Cell Biol 1994; 13(8):821.

Other Notes

Small volumes of anti-FSCN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FSCN1. It may not necessarily be applicable to this product.

NCBI GI #
4507115
NCBI GeneID
6624
NCBI Accession #
NP_003079.1
UniProt Primary Accession #
Q16658
UniProt Secondary Accession #
Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
UniProt Related Accession #
Q16658
GenBank Nucleotide #
NM_003088.3
NCBI Official Full Name
fascin
NCBI Official Synonym Full Names
fascin actin-bundling protein 1
NCBI Official Symbol
FSCN1
NCBI Official Synonym Symbols
HSN; SNL; p55; FAN1
UniProt Protein Name
Fascin
UniProt Synonym Protein Names
55 kDa actin-bundling protein; Singed-like protein; p55
Protein Family
Fascin
UniProt Gene Name
FSCN1
UniProt Synonym Gene Names
FAN1; HSN; SNL
UniProt Entry Name
FSCN1_HUMAN
NCBI Protein Information
fascin

NCBI Summary for FSCN1

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
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UniProt Comments for FSCN1

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
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Research Articles on FSCN1

1. MiR-24 functions as a tumor suppressor in nasopharyngeal carcinoma through targeting FSCN1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FSCN1 antibody

 Products by Pathway  Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-FSCN1 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >28 publications with FSCN1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >11 publications with FSCN1 and Breast Neoplasms
 Urinary Bladder Neoplasms Antibodies  >9 publications with FSCN1 and Urinary Bladder Neoplasms
 Lung Neoplasms Antibodies  >9 publications with FSCN1 and Lung Neoplasms
 Ovarian Neoplasms Antibodies  >8 publications with FSCN1 and Ovarian Neoplasms
 Lung Diseases Antibodies  >6 publications with FSCN1 and Lung Diseases
 Liver Neoplasms Antibodies  >5 publications with FSCN1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >5 publications with FSCN1 and Carcinoma, Hepatocellular
 Pancreatic Neoplasms Antibodies  >4 publications with FSCN1 and Pancreatic Neoplasms
 Liver Diseases Antibodies  >4 publications with FSCN1 and Liver Diseases

Organs/Tissues associated with anti-FSCN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >14 publications with FSCN1 and Lung
 Lymph Node Antibodies  >13 publications with FSCN1 and Lymph Node
 Blood Antibodies  >11 publications with FSCN1 and Blood
 Stomach Antibodies  >10 publications with FSCN1 and Stomach
 Bladder Antibodies  >9 publications with FSCN1 and Bladder
 Brain Antibodies  >7 publications with FSCN1 and Brain
 Liver Antibodies  >5 publications with FSCN1 and Liver
 Bone Antibodies  >5 publications with FSCN1 and Bone
 Vascular Antibodies  >4 publications with FSCN1 and Vascular
 Muscle Antibodies  >4 publications with FSCN1 and Muscle
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