Mouse Anti-MFGE8 Monoclonal Antibody – Cat# MBS439420

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (SPM291)

Milk Fat Globule (MFGE8) Antibody – Mouse Monoclonal

Name: MFGE8 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439420
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439420

MBS439420 is an antibody targeting MFGE8. Also known as Milk Fat Globule (Breast Epithelial Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Milk Fat Globule (MFGE8), Monoclonal Antibody

Full Product Name

Milk Fat Globule (Breast Epithelial Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-MFGE8 antibody

Product Synonym Names

Breast epithelial antigen BA46; EDIL1; HMFG; Lactadherin; Lactadherin precursor; Medin; MFGE8; MFGM; Milk fat globule-EGF factor 8 (MFG-E8)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

SPM291

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

45kDa

OMIM

602281

3D Structure

ModBase 3D Structure for Q08431

Cellular Localization

Cell Surface & Cytoplasmic

Immunogen

Human milk fat globule membrane preparation

Hu-Chromosome Location

15q25

Positive Control

Normal breast or breast carcinoma

Applications Tested/Suitable for anti-MFGE8 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-MFGE8 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-MFGE8 antibody

Larocca D et al. A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains. Cancer Res 1991, 51: 4994-4998 Ceriani RL et al. Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. Proc Natl Acad Sci USA 1977, 74(2):582-6 Corcoran D and Walker RA. Ultrastructural localization of milk fat globule membrane antigens in human breast carcinomas. J Pathol 1990,161(2):161-6 Sterns EE et al. Prognostic significance of the immunohistochemical reaction to human milk fat globule antibodies in node-negative and node-positive breast cancer. Breast Cancer Res Treat 1992, 21(3):193-9 Baildam AD et al. The expression of milk fat globule antigens within human mammary tumours: relationship to steroid hormone receptors and response to endocrine treatment. Eur J Cancer Clin Oncol 1989, 25(3):459-67

Other Notes

Small volumes of anti-MFGE8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MFGE8. It may not necessarily be applicable to this product.

NCBI GI #

167830477

NCBI GeneID

4240

NCBI Accession #

NP_001108086.1

UniProt Primary Accession #

Q08431

UniProt Secondary Accession #

Q53FU9; Q7Z3D2; Q9BTL9; B2R6M7

UniProt Related Accession #

Q08431

GenBank Nucleotide #

NM_001114614.2

NCBI Official Full Name

lactadherin isoform b

NCBI Official Synonym Full Names

milk fat globule-EGF factor 8 protein

NCBI Official Symbol

MFGE8

NCBI Official Synonym Symbols

BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888

UniProt Protein Name

Lactadherin

UniProt Synonym Protein Names

Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8

Protein Family

Lactadherin

UniProt Gene Name

MFGE8

UniProt Synonym Gene Names

MFG-E8

UniProt Entry Name

MFGM_HUMAN

NCBI Protein Information

lactadherin

NCBI Summary for MFGE8

This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

UniProt Comments for MFGE8

MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q25

Cellular Component: extracellular matrix; extracellular region; extracellular space; membrane

Biological Process: cellular protein metabolic process

Research Articles on MFGE8

1. Studied the therapeutic effect of rhMFG-E8 in mouse models of IBD. Treatment with rhMFG-E8 significantly attenuated colitis in both models in a dose-dependent way.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MFGE8 antibody

Products by Pathway	Pathway Diagram
Amyloid Fiber Formation Pathway antibodies	Amyloid Fiber Formation Pathway Diagram
Integrins In Angiogenesis Pathway antibodies	Integrins In Angiogenesis Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram

Diseases associated with anti-MFGE8 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>70 publications with MFGE8 and Inflammation
Disease Models, Animal Antibodies	>29 publications with MFGE8 and Disease Models, Animal
Necrosis Antibodies	>29 publications with MFGE8 and Necrosis
Cardiovascular Diseases Antibodies	>29 publications with MFGE8 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>13 publications with MFGE8 and Breast Neoplasms
Neoplasms, Experimental Antibodies	>8 publications with MFGE8 and Neoplasms, Experimental
Kidney Diseases Antibodies	>5 publications with MFGE8 and Kidney Diseases
Adenocarcinoma Antibodies	>4 publications with MFGE8 and Adenocarcinoma
Liver Diseases Antibodies	>4 publications with MFGE8 and Liver Diseases
Weight Loss Antibodies	>3 publications with MFGE8 and Weight Loss

Organs/Tissues associated with anti-MFGE8 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>76 publications with MFGE8 and Blood
Vascular Antibodies	>40 publications with MFGE8 and Vascular
Spleen Antibodies	>22 publications with MFGE8 and Spleen
Muscle Antibodies	>18 publications with MFGE8 and Muscle
Brain Antibodies	>18 publications with MFGE8 and Brain
Liver Antibodies	>17 publications with MFGE8 and Liver
Bone Antibodies	>16 publications with MFGE8 and Bone
Bone Marrow Antibodies	>14 publications with MFGE8 and Bone Marrow
Intestine Antibodies	>14 publications with MFGE8 and Intestine
Lung Antibodies	>14 publications with MFGE8 and Lung