Mouse Anti-FN1 Monoclonal Antibody – Cat# MBS439441

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).

Fibronectin (FN1) Antibody – Mouse Monoclonal

Name: FN1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439441
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439441

MBS439441 is an antibody targeting FN1. Also known as Fibronectin (8th Type III Repeat) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Fibronectin (FN1), Monoclonal Antibody

Full Product Name

Fibronectin (8th Type III Repeat) Mouse Monoclonal Antibody

Product Gene Name

anti-FN1 antibody

Product Synonym Names

Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

568

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

220kDa (monomer); 440kDa (dimer)

OMIM

135600

3D Structure

ModBase 3D Structure for P02751

Cellular Localization

Connective tissue matrix

Immunogen

High molecular weight proteins secreted by cultivated human fibroblasts

Hu-Chromosome Location

2q35

Positive Control

SW156 cells or Kidney

Applications Tested/Suitable for anti-FN1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-FN1 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-FN1 antibody

Christensen, L., et al., APMIS, 98(7), 615-623 (1990). Christensen, L., et al., APMIS, suppl. 26, 1-39 (1992). Ljubimov AV et. al. Lab Investigation, 1995; 72:461-473

Other Notes

Small volumes of anti-FN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FN1. It may not necessarily be applicable to this product.

NCBI GI #

807066342

NCBI GeneID

2335

NCBI Accession #

NP_001293058.1

UniProt Primary Accession #

P02751

UniProt Secondary Accession #

O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q564H7; Q585T2; B7ZLF0; E9PE77; E9PG29

UniProt Related Accession #

P02751

GenBank Nucleotide #

NM_001306129.1

NCBI Official Full Name

fibronectin isoform 8

NCBI Official Synonym Full Names

fibronectin 1

NCBI Official Symbol

FN1

NCBI Official Synonym Symbols

FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2

UniProt Protein Name

Fibronectin

UniProt Synonym Protein Names

Cold-insoluble globulin; CIG

Protein Family

Fibronectin

UniProt Gene Name

FN1

UniProt Synonym Gene Names

FN; FN; CIG

UniProt Entry Name

FINC_HUMAN

NCBI Protein Information

fibronectin

NCBI Summary for FN1

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

UniProt Comments for FN1

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Research Articles on FN1

1. polymorphisms in rs6725958, rs10202709, and rs35343655 of the Fn gene had no obvious effect on the susceptibility to the development of calcium oxalate stones in the Uighur population, residing in the Xinjiang region of China.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FN1 antibody

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Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram

Diseases associated with anti-FN1 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>86 publications with FN1 and Carcinoma
Fibrosis Antibodies	>40 publications with FN1 and Fibrosis
Inflammation Antibodies	>33 publications with FN1 and Inflammation
Lung Diseases Antibodies	>31 publications with FN1 and Lung Diseases
Lung Neoplasms Antibodies	>25 publications with FN1 and Lung Neoplasms
Cardiovascular Diseases Antibodies	>24 publications with FN1 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>24 publications with FN1 and Breast Neoplasms
Neoplasms, Experimental Antibodies	>23 publications with FN1 and Neoplasms, Experimental
Neoplasm Invasiveness Antibodies	>19 publications with FN1 and Neoplasm Invasiveness
Kidney Diseases Antibodies	>18 publications with FN1 and Kidney Diseases

Organs/Tissues associated with anti-FN1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>69 publications with FN1 and Blood
Muscle Antibodies	>41 publications with FN1 and Muscle
Lung Antibodies	>41 publications with FN1 and Lung
Vascular Antibodies	>37 publications with FN1 and Vascular
Liver Antibodies	>33 publications with FN1 and Liver
Kidney Antibodies	>32 publications with FN1 and Kidney
Bone Antibodies	>31 publications with FN1 and Bone
Embryonic Tissue Antibodies	>23 publications with FN1 and Embryonic Tissue
Brain Antibodies	>22 publications with FN1 and Brain
Connective Tissue Antibodies	>22 publications with FN1 and Connective Tissue