Rat NES1/NUCB2 ELISA Kit – Cat# MBS043945

Nesfatin-1/Nucleobindin 2 (NES1/NUCB2) ELISA Kit for Rat

Name: Rat NES1/NUCB2 ELISA Kit; Nesfatin-1/Nucleobindin 2; MyBioSource
Brand: MyBioSource
SKU: MBS043945
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS043945

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of NES1/NUCB2 (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates,… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Nesfatin-1/Nucleobindin 2 (NES1/NUCB2), ELISA Kit

Full Product Name

Rat Nesfatin-1/Nucleobindin 2 ELISA Kit

Product Gene Name

NES1/NUCB2 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich (Quantitative)

Samples

Undiluted original Rat body fluids, tissue homogenates, secretions or feces samples.

Detection Range

0.25ng/ml-8ng/ml.

Sensitivity

0.1ng/ml.

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

50,196 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (17298286..17353070). Location: 11p15.1

OMIM

608020

3D Structure

ModBase 3D Structure for P80303

Other Notes

Small volumes of NES1/NUCB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NES1/NUCB2 purchase

MBS043945 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nesfatin-1/Nucleobindin 2 (NES1/NUCB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NES1/NUCB2. The ELISA analytical biochemical technique of the MBS043945 kit is based on NES1/NUCB2 antibody-NES1/NUCB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NES1/NUCB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NES1/NUCB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NES1/NUCB2. It may not necessarily be applicable to this product.

NCBI GI #

4826870

NCBI GeneID

4925

NCBI Accession #

NP_005004.1

UniProt Primary Accession #

P80303

UniProt Secondary Accession #

Q8NFT5; A8K642; D3DQX5

UniProt Related Accession #

P80303

GenBank Nucleotide #

NM_005013.2

NCBI Official Full Name

nucleobindin-2

NCBI Official Synonym Full Names

nucleobindin 2

NCBI Official Symbol

NUCB2

NCBI Official Synonym Symbols

NEFA

UniProt Protein Name

Nucleobindin-2

UniProt Synonym Protein Names

DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin

UniProt Gene Name

NUCB2

UniProt Synonym Gene Names

NEFA

UniProt Entry Name

NUCB2_HUMAN

NCBI Protein Information

nucleobindin-2; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4

NCBI Summary for NES1/NUCB2

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

UniProt Comments for NES1/NUCB2

NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Vesicle; Secreted

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nuclear envelope; cytosol

Molecular Function: protein binding; DNA binding; calcium ion binding

Research Articles on NES1/NUCB2

1. nesfatin-1 levels are decreased in large for gestational age compared to appropriate for gestational age fetuses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NES1/NUCB2 elisa kit

Reference Product	PubMed Publications
POMC elisa kit	>8 publications with NES1/NUCB2 and POMC
ERAP1 elisa kit	>2 publications with NES1/NUCB2 and ERAP1

Diseases associated with NES1/NUCB2 elisa kit

Disease Name	Pubmed Publications
Weight Gain Antibodies	>10 publications with NES1/NUCB2 and Weight Gain
Liver Diseases Antibodies	>3 publications with NES1/NUCB2 and Liver Diseases
Neoplasms Antibodies	>3 publications with NES1/NUCB2 and Neoplasms
Disease Models, Animal Antibodies	>3 publications with NES1/NUCB2 and Disease Models, Animal
Necrosis Antibodies	>3 publications with NES1/NUCB2 and Necrosis
Carcinoma Antibodies	>2 publications with NES1/NUCB2 and Carcinoma
Inflammation Antibodies	>2 publications with NES1/NUCB2 and Inflammation
Drug Toxicity Antibodies	>1 publications with NES1/NUCB2 and Drug Toxicity
Learning Disorders Antibodies	>1 publications with NES1/NUCB2 and Learning Disorders
Weight Loss Antibodies	>1 publications with NES1/NUCB2 and Weight Loss

Organs/Tissues associated with NES1/NUCB2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>47 publications with NES1/NUCB2 and Brain
Stomach Antibodies	>14 publications with NES1/NUCB2 and Stomach
Blood Antibodies	>14 publications with NES1/NUCB2 and Blood
Pancreas Antibodies	>13 publications with NES1/NUCB2 and Pancreas
Liver Antibodies	>8 publications with NES1/NUCB2 and Liver
Adipose Tissue Antibodies	>7 publications with NES1/NUCB2 and Adipose Tissue
Pituitary Gland Antibodies	>4 publications with NES1/NUCB2 and Pituitary Gland
Heart Antibodies	>4 publications with NES1/NUCB2 and Heart
Muscle Antibodies	>4 publications with NES1/NUCB2 and Muscle
Intestine Antibodies	>3 publications with NES1/NUCB2 and Intestine