Mouse Anti-ITGAX Monoclonal Antibody – Cat# MBS439537

ITGAX Antibody – Mouse Monoclonal

Name: ITGAX Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439537
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439537

MBS439537 is an antibody targeting ITGAX. Also known as CD11c (Dendritic Cell Marker) Mouse Monoclonal Antibody. Reactive in Human.Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD11c (ITGAX), Monoclonal Antibody

Full Product Name

CD11c (Dendritic Cell Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-ITGAX antibody

Product Synonym Names

CD11 antigen-like family member C; Complement component 3 receptor 4 subunit; Integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); Leu M5 alpha subunit; Leukocyte adhesion glycoprotein p150 95 alpha chain; Myeloid membrane antigen alpha subunit; p150/95

Clonality

Monoclonal

Isotype

IgG2b, kappa

Host

Mouse

Species Reactivity

Human.Others not known.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a protein of 145kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.

Preparation and Storage

Antibody with azide - store at 2 to 8°C.

Molecular Weight

145kDa

OMIM

151510

3D Structure

ModBase 3D Structure for P20702

Cellular Localization

Cell Surface

Immunogen

Recombinant human ITGAX protein fragment (aa 637-827)

Hu-Chromosome Location

16p11.2

Positive Control

Human dendritic cells. Human lymph nodes and tonsils.

Applications Tested/Suitable for anti-ITGAX antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-ITGAX antibody

Flow Cytometry (0.5-1 ug/million cells)
Immunofluorescence (0.5-1ug/mL)
Immunohistology (Formalin-fixed)(1-2ug/mL for 30 min at RT)
Staining of formalin-fixxed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Optimal dilution for specific application should be determined.

Product References and Citations for anti-ITGAX antibody

Nicolaou, F., et al. 2003. CD11c gene expression in hairy cell leukemia is dependent upon activation of the proto-oncogenes Ras and JunD. Blood 101: 4033-4041

Other Notes

Small volumes of anti-ITGAX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGAX. It may not necessarily be applicable to this product.

NCBI GI #

34452173

NCBI GeneID

3687

NCBI Accession #

NP_000878.2

UniProt Primary Accession #

P20702

UniProt Secondary Accession #

Q8IVA6

UniProt Related Accession #

P20702

GenBank Nucleotide #

NM_000887.4

NCBI Official Full Name

integrin alpha-X isoform 2

NCBI Official Synonym Full Names

integrin subunit alpha X

NCBI Official Symbol

ITGAX

NCBI Official Synonym Symbols

CD11C; SLEB6

UniProt Protein Name

Integrin alpha-X

UniProt Synonym Protein Names

CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD_antigen: CD11c

Protein Family

Integrin

UniProt Gene Name

ITGAX

UniProt Synonym Gene Names

CD11C

UniProt Entry Name

ITAX_HUMAN

NCBI Protein Information

integrin alpha-X

NCBI Summary for ITGAX

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

UniProt Comments for ITGAX

ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: cell adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; leukocyte migration; organ morphogenesis

Disease: Systemic Lupus Erythematosus

Research Articles on ITGAX

1. Data suggest that blood neutrophils expressing CD11c antigen and EMR2 protein be considered as potential biomarkers for sepsis and systemic inflammatory response syndrome (SIRS), respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGAX antibody

Reference Product	PubMed Publications
TNF antibody	>8 publications with ITGAX and TNF
ITGB2 antibody	>4 publications with ITGAX and ITGB2
PTPRC antibody	>2 publications with ITGAX and PTPRC

Pathways associated with anti-ITGAX antibody

Products by Pathway	Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
ECM Proteoglycans Pathway antibodies	ECM Proteoglycans Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
Integrin-mediated Cell Adhesion Pathway antibodies	Integrin-mediated Cell Adhesion Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram

Diseases associated with anti-ITGAX antibody

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>669 publications with ITGAX and Immune System Diseases
Inflammation Antibodies	>594 publications with ITGAX and Inflammation
Necrosis Antibodies	>378 publications with ITGAX and Necrosis
Disease Models, Animal Antibodies	>348 publications with ITGAX and Disease Models, Animal
Lung Diseases Antibodies	>181 publications with ITGAX and Lung Diseases
Nervous System Diseases Antibodies	>134 publications with ITGAX and Nervous System Diseases
Cardiovascular Diseases Antibodies	>119 publications with ITGAX and Cardiovascular Diseases
Vascular Diseases Antibodies	>108 publications with ITGAX and Vascular Diseases
Neoplasms, Experimental Antibodies	>107 publications with ITGAX and Neoplasms, Experimental
Liver Diseases Antibodies	>65 publications with ITGAX and Liver Diseases

Organs/Tissues associated with anti-ITGAX antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>2329 publications with ITGAX and Blood
Bone Marrow Antibodies	>580 publications with ITGAX and Bone Marrow
Spleen Antibodies	>487 publications with ITGAX and Spleen
Lymph Node Antibodies	>442 publications with ITGAX and Lymph Node
Lung Antibodies	>316 publications with ITGAX and Lung
Intestine Antibodies	>150 publications with ITGAX and Intestine
Brain Antibodies	>110 publications with ITGAX and Brain
Thymus Antibodies	>79 publications with ITGAX and Thymus
Kidney Antibodies	>73 publications with ITGAX and Kidney
Eye Antibodies	>72 publications with ITGAX and Eye