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Nuclear Antigen Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439546

MBS439546 is an antibody targeting Nuclear Antigen. Also known as Nuclear Antigen (Pan-Nuclear Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nuclear Antigen, Monoclonal Antibody
Full Product Name
Nuclear Antigen (Pan-Nuclear Marker) Mouse Monoclonal Antibody
Product Synonym Names
Nuclear Antigen
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
NM106
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Preparation and Storage
Antibody without azide - store at -20 to -80 degree C. Non-hazardous. No MSDS required.
Immunogen
Nuclei of HL60 cells
Positive Control
All human cells. Tonsil
Applications Tested/Suitable for anti-Nuclear Antigen antibody
Flow Cytometry (Flow), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-Nuclear Antigen antibody
Flow Cytometry (Flow)
Tested Dilution: 1-2ug/million cells

Immunofluorescence (IF)
Tested Dilution: 1-3ug/ml

Immunohistochemistry (IHC)
Tested Dilution: 1-2ug/ml; IHC Protocol; Note: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes

Immunohistochemistry (IHC) of anti-Nuclear Antigen antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Pan-Nuclear Ag Monoclonal Antibody (MBS439546).
Mouse Anti-Nuclear Antigen Monoclonal Antibody – Cat# MBS439546

Immunohistochemistry (IHC) of anti-Nuclear Antigen antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Pan-Nuclear Ag Monoclonal Antibody (MBS439546).
Mouse Anti-Nuclear Antigen Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439546

Immunohistochemistry (IHC) of anti-Nuclear Antigen antibody

Formalin-fixed, paraffin-embedded Rat Colon stained with Pan-Nuclear Ag Monoclonal Antibody (MBS439546).
Mouse Anti-Nuclear Antigen Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439546

Immunohistochemistry (IHC) of anti-Nuclear Antigen antibody

Formalin-fixed, paraffin-embedded Rat Lung stained with Pan-Nuclear Ag Monoclonal Antibody (MBS439546).
Mouse Anti-Nuclear Antigen Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439546

SDS-PAGE of anti-Nuclear Antigen antibody

SDS-PAGE Analysis Purified Pan-Nuclear Antigen Monoclonal Antibody (MBS439546). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-Nuclear Antigen Monoclonal Antibody, SDS-PAGE – Cat# MBS439546

Immunofluoresence (IF) of anti-Nuclear Antigen antibody

Immunofluorescentstaining of PFA-fixedHeLa cells with Pan-Nuclear Antigen Monoclonal Antibody (MBS439546) followed by goat anti-mouse IgG-CF488 (green). Membranes labeled with phalloidin (red).
Mouse Anti-Nuclear Antigen Monoclonal Antibody, Immunofluoresence (IF) – Cat# MBS439546

Product References and Citations for anti-Nuclear Antigen antibody

Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseas

Other Notes

Small volumes of anti-Nuclear Antigen antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Nuclear Antigen. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
nuclear antigen, partial
UniProt Protein Name
Nuclear antigen
UniProt Synonym Protein Names
Nuclear antigen
Protein Family
UniProt Entry Name
A6N0I9_ORYSI

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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