Mouse Anti-CD55 Monoclonal Antibody – Cat# MBS439570

CD55 Antibody – Mouse Monoclonal

Name: CD55 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439570
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439570

MBS439570 is an antibody targeting CD55. Also known as CD55 / Decay Accelerating Factor (DAF) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD55 / Decay Accelerating Factor (DAF), Monoclonal Antibody

Full Product Name

CD55 / Decay Accelerating Factor (DAF) Mouse Monoclonal Antibody

Product Gene Name

anti-CD55 antibody

Product Synonym Names

CD55; CD55 Cromer blood group system; Complement decay-accelerating factor; CROM; DAF; Daf-GPI; Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

143-30

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

70kDa

OMIM

125240

3D Structure

ModBase 3D Structure for P08174

Cellular Localization

Cell Surface

Immunogen

PHA stimulated human PBL

Hu-Chromosome Location

1q32

Positive Control

Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes. Human lymph nodes and tonsils.

Applications Tested/Suitable for anti-CD55 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-CD55 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Frozen) (0.5-1ug/ml for 30 minutes at RT)

Product References and Citations for anti-CD55 antibody

Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York and London, 1997.Koretz, K. et al., Decay-accelerating factor (DAF, CD55) in normal colorectal mucosa, adenomas and carcinomas. British J. Cancer 66: 810-814, (1992). Knapp, W. et al., Leucocyte typing IV, pp 541, 694-697, 1088. Oxford Univ

Other Notes

Small volumes of anti-CD55 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD55. It may not necessarily be applicable to this product.

NCBI GI #

10835143

NCBI GeneID

1604

NCBI Accession #

NP_000565.1

UniProt Primary Accession #

P08174

UniProt Secondary Accession #

P09679; P78361; Q14UF2; Q14UF3; Q14UF4; Q14UF5; Q14UF6; B1AP14; D3DT83; D3DT84; E7ER69

UniProt Related Accession #

P08174

GenBank Nucleotide #

NM_000574.4

NCBI Official Full Name

complement decay-accelerating factor isoform 1 preproprotein

NCBI Official Synonym Full Names

CD55 molecule (Cromer blood group)

NCBI Official Symbol

CD55

NCBI Official Synonym Symbols

CR; TC; DAF; CROM

UniProt Protein Name

Complement decay-accelerating factor

UniProt Synonym Protein Names

CD_antigen: CD55

Protein Family

Complement decay-accelerating factor

UniProt Gene Name

CD55

UniProt Synonym Gene Names

CR; DAF

UniProt Entry Name

DAF_HUMAN

NCBI Protein Information

complement decay-accelerating factor

NCBI Summary for CD55

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]

UniProt Comments for CD55

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.

Research Articles on CD55

1. present study suggested that the expressions of CD97 antigen and decay accelerating factor DAF were both upregulated in human cervical squamous cell carcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD55 antibody

Reference Product	PubMed Publications
CD59 antibody	>883 publications with CD55 and CD59
CD97 antibody	>52 publications with CD55 and CD97
EMR2 antibody	>10 publications with CD55 and EMR2
LCK antibody	>8 publications with CD55 and LCK

Pathways associated with anti-CD55 antibody

Products by Pathway	Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
COPI-mediated Anterograde Transport Pathway antibodies	COPI-mediated Anterograde Transport Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
Complement Activation, Classical Pathway antibodies	Complement Activation, Classical Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement Cascade Pathway antibodies	Complement Cascade Pathway Diagram
ER To Golgi Anterograde Transport Pathway antibodies	ER To Golgi Anterograde Transport Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD55 antibody

Disease Name	Pubmed Publications
Anemia Antibodies	>354 publications with CD55 and Anemia
Inflammation Antibodies	>151 publications with CD55 and Inflammation
Necrosis Antibodies	>87 publications with CD55 and Necrosis
Disease Models, Animal Antibodies	>75 publications with CD55 and Disease Models, Animal
Kidney Diseases Antibodies	>65 publications with CD55 and Kidney Diseases
Nervous System Diseases Antibodies	>60 publications with CD55 and Nervous System Diseases
Lung Diseases Antibodies	>34 publications with CD55 and Lung Diseases
Heart Diseases Antibodies	>26 publications with CD55 and Heart Diseases
Breast Neoplasms Antibodies	>25 publications with CD55 and Breast Neoplasms
Brain Diseases Antibodies	>24 publications with CD55 and Brain Diseases

Organs/Tissues associated with anti-CD55 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>948 publications with CD55 and Blood
Vascular Antibodies	>214 publications with CD55 and Vascular
Kidney Antibodies	>159 publications with CD55 and Kidney
Bone Antibodies	>137 publications with CD55 and Bone
Heart Antibodies	>133 publications with CD55 and Heart
Bone Marrow Antibodies	>126 publications with CD55 and Bone Marrow
Muscle Antibodies	>92 publications with CD55 and Muscle
Lung Antibodies	>89 publications with CD55 and Lung
Liver Antibodies	>86 publications with CD55 and Liver
Ovary Antibodies	>51 publications with CD55 and Ovary