Mouse Anti-ALPP Monoclonal Antibody – Cat# MBS439657

Formalin-fixed, paraffin-embedded human Placenta stained with PLAP Monoclonal Antibody (GM022).

Alkaline Phosphatase / PLAP (ALPP) Antibody – Mouse Monoclonal

Name: ALPP Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439657
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439657

MBS439657 is an antibody targeting ALPP. Also known as Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Alkaline Phosphatase / PLAP (ALPP), Monoclonal Antibody

Full Product Name

Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-ALPP antibody

Product Synonym Names

Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; ALP; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP-1; PLAP1

Clonality

Monoclonal

Isotype

IgG2b, kappa

Clone Number

GM022

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the gestational trophoblastic disease.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

70kDa

OMIM

171800

3D Structure

ModBase 3D Structure for P05187

Cellular Localization

Cytoplasmic and Cell Surface

Immunogen

Recombinant human PLAP protein

Hu-Chromosome Location

2q37.1

Positive Control

HepG2 cells. Placenta or seminoma

Applications Tested/Suitable for anti-ALPP antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-ALPP antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)

Product References and Citations for anti-ALPP antibody

Wick, MR, et al. 1987; Hum Pathol. 18(9):946-54.

Other Notes

Small volumes of anti-ALPP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALPP. It may not necessarily be applicable to this product.

NCBI GI #

94721246

NCBI GeneID

250

NCBI Accession #

NP_001623.3

UniProt Primary Accession #

P05187

UniProt Secondary Accession #

P05188; P06861; Q53S78; Q96DB7

UniProt Related Accession #

P05187

GenBank Nucleotide #

NM_001632.4

NCBI Official Full Name

alkaline phosphatase, placental type preproprotein

NCBI Official Synonym Full Names

alkaline phosphatase, placental

NCBI Official Symbol

ALPP

NCBI Official Synonym Symbols

ALP; PALP; PLAP; PLAP-1

UniProt Protein Name

Alkaline phosphatase, placental type

UniProt Synonym Protein Names

Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1

Protein Family

Alkaline phosphatase

UniProt Gene Name

ALPP

UniProt Synonym Gene Names

PLAP; PLAP-1

UniProt Entry Name

PPB1_HUMAN

NCBI Protein Information

alkaline phosphatase, placental type

NCBI Summary for ALPP

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

Research Articles on ALPP

1. Quantum-mechanical computational methods were employed to study the catalytic mechanism of human placental AP (PLAP). An active-site model was used, constructed on the basis of the X-ray crystal structure of the enzyme.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ALPP antibody

Products by Pathway	Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram
Intra-Golgi And Retrograde Golgi-to-ER Traffic Pathway antibodies	Intra-Golgi And Retrograde Golgi-to-ER Traffic Pathway Diagram
Intra-Golgi Traffic Pathway antibodies	Intra-Golgi Traffic Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Vesicle-mediated Transport Pathway antibodies	Vesicle-mediated Transport Pathway Diagram
Pyridoxal 5'-phosphate Salvage Pathway antibodies	Pyridoxal 5'-phosphate Salvage Pathway Diagram

Diseases associated with anti-ALPP antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>29 publications with ALPP and Adenocarcinoma
Pregnancy Complications Antibodies	>23 publications with ALPP and Pregnancy Complications
Lung Diseases Antibodies	>12 publications with ALPP and Lung Diseases
Disease Models, Animal Antibodies	>12 publications with ALPP and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>7 publications with ALPP and Cell Transformation, Neoplastic
Liver Diseases Antibodies	>6 publications with ALPP and Liver Diseases
Neoplasms, Experimental Antibodies	>5 publications with ALPP and Neoplasms, Experimental
Pain Antibodies	>4 publications with ALPP and Pain
Necrosis Antibodies	>3 publications with ALPP and Necrosis
Hyperplasia Antibodies	>3 publications with ALPP and Hyperplasia

Organs/Tissues associated with anti-ALPP antibody

Organ/Tissue Name	Pubmed Publications
Placenta Antibodies	>89 publications with ALPP and Placenta
Intestine Antibodies	>10 publications with ALPP and Intestine
Heart Antibodies	>8 publications with ALPP and Heart
Uterus Antibodies	>7 publications with ALPP and Uterus
Amniotic Fluid Antibodies	>1 publications with ALPP and Amniotic Fluid