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Pgp9.5 / Uchl1 (UCHL1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439747

MBS439747 is an antibody targeting UCHL1. Also known as PGP9.5 / UchL1 (pan-Neuronal Marker) Mouse Monoclonal Antibody. Reactive in Human, Rat.Shows a broad species reactivity. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PGP9.5 / UchL1, Monoclonal Antibody
Full Product Name
PGP9.5 / UchL1 (pan-Neuronal Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-UCHL1 antibody
Product Synonym Names
Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
UCHL1/775
Host
Mouse
Species Reactivity
Human, Rat.
Shows a broad species reactivity
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
20-30kDa
OMIM
191342
3D Structure
ModBase 3D Structure for P09936
Cellular Localization
Cytoplasmic. Endoplasmic Reticulum membrane
Immunogen
Recombinant human UCHL1 protein
Hu-Chromosome Location
4p13
Positive Control
Cerebellum
Applications Tested/Suitable for anti-UCHL1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-UCHL1 antibody
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-UCHL1 antibody

Formalin-fixed, paraffin-embedded Rat Testis stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).
Mouse Anti-UCHL1 Monoclonal Antibody – Cat# MBS439747

Immunohistochemistry (IHC) of anti-UCHL1 antibody

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).
Mouse Anti-UCHL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439747

Product References and Citations for anti-UCHL1 antibody

Wilkinson, K.D., et al. 1989. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science 246: 670-672.

Other Notes

Small volumes of anti-UCHL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UCHL1. It may not necessarily be applicable to this product.

NCBI GI #
21361091
NCBI GeneID
7345
NCBI Accession #
NP_004172.2
UniProt Primary Accession #
P09936
UniProt Secondary Accession #
Q4W5K6; Q71UM0
UniProt Related Accession #
P09936
GenBank Nucleotide #
NM_004181.4
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names
Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
Protein Family
Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
UniProt Entry Name
UCHL1_HUMAN
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1

NCBI Summary for UCHL1

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
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UniProt Comments for UCHL1

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Protease; Ubiquitin conjugating system; EC 3.4.19.12; Cell development/differentiation; Ligase

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: cytoplasm; nucleoplasm; plasma membrane

Molecular Function: alpha-2A adrenergic receptor binding; cysteine-type endopeptidase activity; omega peptidase activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: negative regulation of MAP kinase activity; protein deubiquitination; regulation of macroautophagy

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant
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Research Articles on UCHL1

1. Serum UCH-l1 levels serve as a novel biomarker for neuronal damage from white matter lesions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UCHL1 antibody

 Reference Product  PubMed Publications
 SNCA antibody  >30 publications with UCHL1 and SNCA
 CDKN2A antibody  >4 publications with UCHL1 and CDKN2A
 TP53 antibody  >4 publications with UCHL1 and TP53
 HSP90AA1 antibody  >2 publications with UCHL1 and HSP90AA1
 COPS5 antibody  >1 publications with UCHL1 and COPS5
 HSPA8 antibody  >1 publications with UCHL1 and HSPA8

Pathways associated with anti-UCHL1 antibody

 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram

Diseases associated with anti-UCHL1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >245 publications with UCHL1 and Nervous System Diseases
 Brain Diseases Antibodies  >174 publications with UCHL1 and Brain Diseases
 Inflammation Antibodies  >48 publications with UCHL1 and Inflammation
 Disease Models, Animal Antibodies  >43 publications with UCHL1 and Disease Models, Animal
 Peripheral Nervous System Diseases Antibodies  >38 publications with UCHL1 and Peripheral Nervous System Diseases
 Cardiovascular Diseases Antibodies  >35 publications with UCHL1 and Cardiovascular Diseases
 Necrosis Antibodies  >25 publications with UCHL1 and Necrosis
 Nerve Degeneration Antibodies  >22 publications with UCHL1 and Nerve Degeneration
 Brain Injuries Antibodies  >19 publications with UCHL1 and Brain Injuries
 Lung Neoplasms Antibodies  >18 publications with UCHL1 and Lung Neoplasms

Organs/Tissues associated with anti-UCHL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >221 publications with UCHL1 and Nerve
 Brain Antibodies  >144 publications with UCHL1 and Brain
 Skin Antibodies  >104 publications with UCHL1 and Skin
 Muscle Antibodies  >66 publications with UCHL1 and Muscle
 Lymph Antibodies  >66 publications with UCHL1 and Lymph
 Lymph Node Antibodies  >64 publications with UCHL1 and Lymph Node
 Bone Antibodies  >64 publications with UCHL1 and Bone
 Vascular Antibodies  >60 publications with UCHL1 and Vascular
 Lung Antibodies  >43 publications with UCHL1 and Lung
 Bone Marrow Antibodies  >37 publications with UCHL1 and Bone Marrow
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