• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

UACA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439765

MBS439765 is an antibody targeting UACA. Also known as UACA / Nucling (Nuclear Membrane Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UACA / Nucling, Monoclonal Antibody
Full Product Name
UACA / Nucling (Nuclear Membrane Marker) Mouse Monoclonal Antibody
Product Gene Name
Product Synonym Names
Nuclear membrane binding protein; Uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA); NUCLING
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
AE-5
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
160kDa
OMIM
612516
3D Structure
ModBase 3D Structure for Q9BZF9
Cellular Localization
Nuclear membrane
Immunogen
Nuclei of myeloid leukemia biopsy cells
Hu-Chromosome Location
15q22-q24
Positive Control
HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.
Applications Tested/Suitable for anti-UACA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-UACA antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1.0ug/ml)
Western Blot (0.5-1ug/ml)

Western Blot (WB) of anti-UACA antibody

Western Blot of A549 Cell Lysate using UACA / Nucling Monoclonal Antibody (AE-5)
Mouse Anti-UACA Monoclonal Antibody – Cat# MBS439765

Product References and Citations for anti-UACA antibody

Yamada, K., et al. 2001. Identification of a novel autoantigen UACA in patients with panuveitis. Biochem. Biophys. Res. Commun. 280: 1169-1176. Ohkura, T., et al. 2004. Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves disease. Biochem. Biophys. Res. Commun. 321: 432-440

Other Notes

Small volumes of anti-UACA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UACA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
uveal autoantigen with coiled-coil domains and ankyrin repeats isoform 2
NCBI Official Synonym Full Names
uveal autoantigen with coiled-coil domains and ankyrin repeats
NCBI Official Symbol
UACA
NCBI Official Synonym Symbols
NUCLING
UniProt Protein Name
Uveal autoantigen with coiled-coil domains and ankyrin repeats
UniProt Gene Name
UACA
UniProt Synonym Gene Names
KIAA1561
UniProt Entry Name
UACA_HUMAN
NCBI Protein Information
uveal autoantigen with coiled-coil domains and ankyrin repeats

UniProt Comments for UACA

UACA: Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 15q22-q24

Cellular Component: cytoplasm; mitochondrion; nucleus

Molecular Function: protein binding
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000