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ACTA2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439779

MBS439779 is an antibody targeting ACTA2. Also known as Actin, Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody. Reactive in Human, Rat.Predicted to show a broad reactivity. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Actin (ACTA2), Monoclonal Antibody
Full Product Name
Actin, Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-ACTA2 antibody
Product Synonym Names
ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
ACTA2/791
Host
Mouse
Species Reactivity
Human, Rat.
Predicted to show a broad reactivity
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
42kDa
OMIM
102620
3D Structure
ModBase 3D Structure for P62736
Cellular Localization
Cytoplasmic
Immunogen
Recombinant full-length human ACTA2 protein
Hu-Chromosome Location
10q23.31
Positive Control
Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Applications Tested/Suitable for anti-ACTA2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ACTA2 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-ACTA2 antibody

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).
Mouse Anti-ACTA2 Monoclonal Antibody – Cat# MBS439779

Immunohistochemistry (IHC) of anti-ACTA2 antibody

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).
Mouse Anti-ACTA2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439779

Immunohistochemistry (IHC) of anti-ACTA2 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).
Mouse Anti-ACTA2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439779

Immunohistochemistry (IHC) of anti-ACTA2 antibody

Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).
Mouse Anti-ACTA2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439779

Product References and Citations for anti-ACTA2 antibody

Gown, A.M., et al. 1985. A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes. J. Cell Biol. 100: 807-813.

Other Notes

Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.

NCBI GI #
213688375
NCBI GeneID
59
NCBI Accession #
NP_001135417.1
UniProt Primary Accession #
P62736
UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4
UniProt Related Accession #
P62736
GenBank Nucleotide #
NM_001141945.2
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Protein Family
Actin
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_HUMAN
NCBI Protein Information
actin, aortic smooth muscle

NCBI Summary for ACTA2

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
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UniProt Comments for ACTA2

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Contractile

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: cytoplasm; cytosol; extracellular space; filopodium; lamellipodium; protein complex

Molecular Function: protein kinase binding

Biological Process: muscle contraction; response to virus

Disease: Aortic Aneurysm, Familial Thoracic 6; Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome
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Research Articles on ACTA2

1. NGF has a role in modulating trkANGFR/p75NTR in alphaSMA-expressing conjunctival fibroblasts from human ocular cicatricial pemphigoid

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACTA2 antibody

 Reference Product  PubMed Publications
 MYH11 antibody  >50 publications with ACTA2 and MYH11
 SRF antibody  >20 publications with ACTA2 and SRF
 MYOCD antibody  >10 publications with ACTA2 and MYOCD
 TPM3 antibody  >8 publications with ACTA2 and TPM3
 TPM1 antibody  >2 publications with ACTA2 and TPM1
 UBC antibody  >2 publications with ACTA2 and UBC
 TPM2 antibody  >1 publications with ACTA2 and TPM2

Pathways associated with anti-ACTA2 antibody

 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Smooth Muscle Contraction Pathway antibodies  Smooth Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram

Diseases associated with anti-ACTA2 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >348 publications with ACTA2 and Fibrosis
 Cardiovascular Diseases Antibodies  >155 publications with ACTA2 and Cardiovascular Diseases
 Inflammation Antibodies  >114 publications with ACTA2 and Inflammation
 Liver Diseases Antibodies  >105 publications with ACTA2 and Liver Diseases
 Disease Models, Animal Antibodies  >88 publications with ACTA2 and Disease Models, Animal
 Liver Cirrhosis Antibodies  >75 publications with ACTA2 and Liver Cirrhosis
 Kidney Diseases Antibodies  >57 publications with ACTA2 and Kidney Diseases
 Heart Diseases Antibodies  >49 publications with ACTA2 and Heart Diseases
 Aortic Aneurysm, Thoracic Antibodies  >45 publications with ACTA2 and Aortic Aneurysm, Thoracic
 Breast Neoplasms Antibodies  >40 publications with ACTA2 and Breast Neoplasms

Organs/Tissues associated with anti-ACTA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >840 publications with ACTA2 and Muscle
 Vascular Antibodies  >259 publications with ACTA2 and Vascular
 Blood Antibodies  >191 publications with ACTA2 and Blood
 Lung Antibodies  >150 publications with ACTA2 and Lung
 Eye Antibodies  >141 publications with ACTA2 and Eye
 Liver Antibodies  >134 publications with ACTA2 and Liver
 Connective Tissue Antibodies  >131 publications with ACTA2 and Connective Tissue
 Kidney Antibodies  >104 publications with ACTA2 and Kidney
 Skin Antibodies  >88 publications with ACTA2 and Skin
 Heart Antibodies  >70 publications with ACTA2 and Heart
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