Mouse Anti-ARG1 Monoclonal Antibody – Cat# MBS439804

Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with ARG1 Monoclonal Antibody (ARG1/1126).

Arginase 1 (ARG1) Antibody – Mouse Monoclonal

Name: ARG1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439804
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439804

MBS439804 is an antibody targeting ARG1. Also known as Arginase 1 (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Arginase 1 (ARG1), Monoclonal Antibody

Full Product Name

Arginase 1 (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-ARG1 antibody

Product Synonym Names

Arginase 1; ARG1; liver-type arginase; type I arginase

Clonality

Monoclonal

Isotype

IgG3, kappa

Clone Number

ARG1/1126

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

35-38kDa

OMIM

207800

3D Structure

ModBase 3D Structure for P05089

Cellular Localization

Cytoplasmic

Immunogen

Recombinant fragment (87 Amino acid residues around aa 1-150) of human ARG1 protein

Hu-Chromosome Location

6q23

Positive Control

293T cells. Hepatocellular Carcinoma (HCC).

Applications Tested/Suitable for anti-ARG1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-ARG1 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-ARG1 antibody

Diez, A., et al. 1994. Immunological identity of the two different molecular mass constitutive subunits of liver arginase. Biol. Chem. Hoppe Seyler 375: 537-541

Other Notes

Small volumes of anti-ARG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARG1. It may not necessarily be applicable to this product.

NCBI GI #

10947139

NCBI GeneID

383

NCBI Accession #

NP_000036.2

UniProt Primary Accession #

P05089

UniProt Secondary Accession #

Q5JWT5; Q5JWT6; Q8TE72; Q9BS50; A6NEA0

UniProt Related Accession #

P05089

GenBank Nucleotide #

NM_000045.3

NCBI Official Full Name

arginase-1 isoform 2

NCBI Official Synonym Full Names

arginase 1

NCBI Official Symbol

ARG1

UniProt Protein Name

Arginase-1

UniProt Synonym Protein Names

Liver-type arginase; Type I arginase

Protein Family

Arginase

UniProt Gene Name

ARG1

UniProt Entry Name

ARGI1_HUMAN

NCBI Protein Information

arginase-1

NCBI Summary for ARG1

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for ARG1

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.3.1; Hydrolase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: arginase activity

Biological Process: arginine catabolic process; urea cycle

Disease: Argininemia

Research Articles on ARG1

1. Arginase inhibition arrests human pulmonary artery smooth muscle cells in the G1/G0-phase under hypoxic conditions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ARG1 antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
IL4-mediated Signaling Events Pathway antibodies	IL4-mediated Signaling Events Pathway Diagram

Diseases associated with anti-ARG1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>177 publications with ARG1 and Inflammation
Disease Models, Animal Antibodies	>98 publications with ARG1 and Disease Models, Animal
Lung Diseases Antibodies	>71 publications with ARG1 and Lung Diseases
Necrosis Antibodies	>55 publications with ARG1 and Necrosis
Cardiovascular Diseases Antibodies	>51 publications with ARG1 and Cardiovascular Diseases
Immune System Diseases Antibodies	>49 publications with ARG1 and Immune System Diseases
Fibrosis Antibodies	>33 publications with ARG1 and Fibrosis
Asthma Antibodies	>31 publications with ARG1 and Asthma
Neoplasms, Experimental Antibodies	>28 publications with ARG1 and Neoplasms, Experimental
Liver Diseases Antibodies	>28 publications with ARG1 and Liver Diseases

Organs/Tissues associated with anti-ARG1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>283 publications with ARG1 and Blood
Muscle Antibodies	>118 publications with ARG1 and Muscle
Lung Antibodies	>105 publications with ARG1 and Lung
Brain Antibodies	>102 publications with ARG1 and Brain
Liver Antibodies	>80 publications with ARG1 and Liver
Bone Marrow Antibodies	>55 publications with ARG1 and Bone Marrow
Spleen Antibodies	>35 publications with ARG1 and Spleen
Eye Antibodies	>30 publications with ARG1 and Eye
Skin Antibodies	>23 publications with ARG1 and Skin
Uterus Antibodies	>11 publications with ARG1 and Uterus