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MUC18 /CD146 / MCAM (Melanoma Cell Adhesion Molecule) (MCAM) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439806

MBS439806 is an antibody targeting MCAM. Also known as MUC18 /CD146 / MCAM (Melanoma Cell Adhesion Molecule) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MUC18 /CD146 / MCAM (Melanoma Cell Adhesion Molecule), Monoclonal Antibody
Full Product Name
MUC18 /CD146 / MCAM (Melanoma Cell Adhesion Molecule) Mouse Monoclonal Antibody
Product Gene Name
anti-MCAM antibody
Product Synonym Names
Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
MUC18/1130
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
130kDa
OMIM
155735
3D Structure
ModBase 3D Structure for P43121
Cellular Localization
Cell Surface
Immunogen
Recombinant human MUC18 protein
Hu-Chromosome Location
11q23.3
Positive Control
A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
Applications Tested/Suitable for anti-MCAM antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-MCAM antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-MCAM antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)
Mouse Anti-MCAM Monoclonal Antibody – Cat# MBS439806

Immunohistochemistry (IHC) of anti-MCAM antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)
Mouse Anti-MCAM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439806

Immunohistochemistry (IHC) of anti-MCAM antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with MUC18 Monoclonal Antibody (MUC18/1130)
Mouse Anti-MCAM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439806

Product References and Citations for anti-MCAM antibody

Pruszak J et al. Stem Cells 25:2257-68 (2007

Other Notes

Small volumes of anti-MCAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCAM. It may not necessarily be applicable to this product.

NCBI GI #
71274107
NCBI GeneID
4162
NCBI Accession #
NP_006491.2
UniProt Primary Accession #
P43121
UniProt Secondary Accession #
O95812; Q59E86; Q6PHR3; Q6ZTR2
UniProt Related Accession #
P43121
GenBank Nucleotide #
NM_006500.2
NCBI Official Full Name
cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
UniProt Synonym Protein Names
Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146
Protein Family
Cell surface glycoprotein
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18
UniProt Entry Name
MUC18_HUMAN
NCBI Protein Information
cell surface glycoprotein MUC18

UniProt Comments for MCAM

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: external side of plasma membrane; focal adhesion

Biological Process: anatomical structure morphogenesis; angiogenesis; glomerular filtration
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Research Articles on MCAM

1. CD146 is a novel and useful marker for predicting senescence in human umbilical cord blood-derived Mesenchymal stem cells (hUCB-MSCs), and CD146 can potentially be applied in quality-control assessments of hUCB-MSC-based therapy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCAM antibody

 Reference Product  PubMed Publications
 FGF2 antibody  >8 publications with MCAM and FGF2
 PTK2 antibody  >4 publications with MCAM and PTK2
 TGFB1 antibody  >4 publications with MCAM and TGFB1
 ATF3 antibody  >2 publications with MCAM and ATF3
 LGALS1 antibody  >2 publications with MCAM and LGALS1
 ESR1 antibody  >2 publications with MCAM and ESR1
 CYP1B1 antibody  >1 publications with MCAM and CYP1B1
 EDN1 antibody  >1 publications with MCAM and EDN1

Diseases associated with anti-MCAM antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >42 publications with MCAM and Inflammation
 Lung Diseases Antibodies  >32 publications with MCAM and Lung Diseases
 Disease Models, Animal Antibodies  >30 publications with MCAM and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >27 publications with MCAM and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >26 publications with MCAM and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >26 publications with MCAM and Adenocarcinoma
 Lung Neoplasms Antibodies  >25 publications with MCAM and Lung Neoplasms
 Breast Neoplasms Antibodies  >23 publications with MCAM and Breast Neoplasms
 Nervous System Diseases Antibodies  >20 publications with MCAM and Nervous System Diseases
 Liver Diseases Antibodies  >17 publications with MCAM and Liver Diseases

Organs/Tissues associated with anti-MCAM antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >149 publications with MCAM and Blood
 Vascular Antibodies  >117 publications with MCAM and Vascular
 Bone Antibodies  >56 publications with MCAM and Bone
 Skin Antibodies  >55 publications with MCAM and Skin
 Lung Antibodies  >50 publications with MCAM and Lung
 Embryonic Tissue Antibodies  >42 publications with MCAM and Embryonic Tissue
 Bone Marrow Antibodies  >39 publications with MCAM and Bone Marrow
 Muscle Antibodies  >32 publications with MCAM and Muscle
 Brain Antibodies  >25 publications with MCAM and Brain
 Liver Antibodies  >24 publications with MCAM and Liver
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