Mouse Anti-KRT Monoclonal Antibody – Cat# MBS439827

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, pan Monoclonal Antibody cocktail (PAN-CK).

Cytokeratin, Pan (KRT) Antibody – Mouse Monoclonal

Name: KRT Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS439827
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439827

MBS439827 is an antibody targeting KRT. Also known as Cytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody. Reactive in Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin, pan (KRT), Monoclonal Antibody

Full Product Name

Cytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-KRT antibody

Product Synonym Names

K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

Clonality

Monoclonal

Isotype

IgG, kappa

Clone Number

PAN-CK (Cocktail)

Host

Mouse

Species Reactivity

Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18) and 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

40-67kDa (Multiple)

OMIM

611158

3D Structure

ModBase 3D Structure for Q7Z794

Cellular Localization

Cytoplasmic

Immunogen

Human epidermal keratin

Hu-Chromosome Location

17q21.2 (KRT77) & 12q13.13 (KRT76)

Positive Control

Skin, Adeno- or Squamous carcinomas

Applications Tested/Suitable for anti-KRT antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Application Notes for anti-KRT antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1ug/ml for 2 hours at RT)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-KRT antibody

Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. Tseng SCG et. al. Cell 1982; 30361

Other Notes

Small volumes of anti-KRT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT. It may not necessarily be applicable to this product.

NCBI GI #

194018511

NCBI GeneID

374454

NCBI Accession #

NP_778253.2

UniProt Primary Accession #

Q7Z794

UniProt Secondary Accession #

Q7RTS8

UniProt Related Accession #

Q7Z794

GenBank Nucleotide #

NM_175078.2

NCBI Official Full Name

keratin, type II cytoskeletal 1b

NCBI Official Synonym Full Names

keratin 77

NCBI Official Symbol

KRT77

NCBI Official Synonym Symbols

K1B; KRT1B

UniProt Protein Name

Keratin, type II cytoskeletal 1b

UniProt Synonym Protein Names

Cytokeratin-1B; CK-1B; Keratin-77; K77; Type-II keratin Kb39

UniProt Gene Name

KRT77

UniProt Synonym Gene Names

KRT1B; CK-1B; K77

UniProt Entry Name

K2C1B_HUMAN

NCBI Protein Information

keratin, type II cytoskeletal 1b

NCBI Summary for KRT

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009]

UniProt Comments for KRT

K77: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009]

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoskeleton

Research Articles on KRT

1. Epithelial keratin, K1b, is exclusively expressed in luminal duct cells of eccrine sweat glands.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-KRT antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>5 publications with KRT and Skin
Uncharacterized Tissue Antibodies	>1 publications with KRT and Uncharacterized Tissue