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CD21 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439888

MBS439888 is an antibody targeting CD21. Also known as CD21 (Mature B-Cell & Follicular Dendritic Cell Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD21, Monoclonal Antibody
Full Product Name
CD21 (Mature B-Cell & Follicular Dendritic Cell Marker)
Product Gene Name
Product Synonym Gene Name
CR2
Product Synonym Names
CD21; Complement C3d receptor (C3DR); Complement Receptor type 2 (CR2); CVID7; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR; SLEB9
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
CR2/1953
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) / CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
140kDa
3D Structure
ModBase 3D Structure for P20023
Immunogen
Recombinant human CR2 (CD21) protein fragment (around aa 44-196)
Cellular Localization
Cell Surface
Hu-chromosome Location
1q32
Positive Control
WEHI-231 cells and Tonsil
Applications Tested/Suitable for anti-CD21 antibody
Immunohistochemistry (IHC) Formalin
Application Notes for anti-CD21 antibody
IHC-F: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Immunohistochemistry (IHC) of anti-CD21 antibody

Formalin-fixed, paraffin-embedded Human Follicular Dendritic Cell Sarcoma stained with CD21 / CR2 Mouse Monoclonal Antibody (CR2/1953).
Mouse Anti-CD21 Monoclonal Antibody – Cat# MBS439888

Immunohistochemistry (IHC) of anti-CD21 antibody

Formalin-fixed, paraffin-embedded Human Tonsil stained with CD21 / CR2 Mouse Monoclonal Antibody (CR2/1953).
Mouse Anti-CD21 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439888

SDS-Page of anti-CD21 antibody

SDS-PAGE Analysis Purified CD21 / CR2 Mouse Monoclonal Antibody (CR2/1953). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-CD21 Monoclonal Antibody, SDS-Page – Cat# MBS439888

Testing Data of anti-CD21 antibody

Analysis of Protein Array containing more than 19, 000 full-length human proteins using CD21 Mouse Monoclonal Antibody (CR2/1953).
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-CD21 Monoclonal Antibody, Testing Data – Cat# MBS439888

Product References and Citations for anti-CD21 antibody

Otsuka, M., et al. 2004. Role of CD21 antigen in diffuse large B-cell lymphoma and its clinical significance. Br. J. Haematol. 127: 416-424.

Other Notes

Small volumes of anti-CD21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD21. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
complement receptor type 2 isoform 1
NCBI Official Synonym Full Names
complement C3d receptor 2
NCBI Official Symbol
CR2
NCBI Official Synonym Symbols
CR; C3DR; CD21; CVID7; SLEB9
UniProt Protein Name
Complement receptor type 2
UniProt Synonym Protein Names
Complement C3d receptor; Epstein-Barr virus receptor; EBV receptor; CD_antigen: CD21
Protein Family
UniProt Gene Name
CR2
UniProt Synonym Gene Names
C3DR; Cr2; EBV receptor
NCBI Protein Information
complement receptor type 2

NCBI Summary for CD21

This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
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UniProt Comments for CD21

Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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