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NSE gamma Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439932

MBS439932 is an antibody targeting NSE gamma. Also known as NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker). Reactive in Human, Mouse, Rat. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NSE gamma (Neuron Specific Enolase, gamma), Monoclonal Antibody
Full Product Name
NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)
Product Gene Name
Product Synonym Gene Name
ENO2
Product Synonym Names
2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
ENO2/1375
Host
Mouse
Species Reactivity
Human, Mouse, Rat. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
~50kDa
3D Structure
ModBase 3D Structure for P09104
Immunogen
A synthetic peptide corresponding to aa416-433 of human NSE gamma
Cellular Localization
Cytoplasmic
Hu-chromosome Location
12p13
Positive Control
HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
Applications Tested/Suitable for anti-NSE gamma antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-NSE gamma antibody
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.1-0.2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-NSE gamma antibody

Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1375).
Mouse Anti-NSE gamma Monoclonal Antibody – Cat# MBS439932

Immunohistochemistry (IHC) of anti-NSE gamma antibody

Formalin-fixed, paraffin-embedded Human Cerebellum stained with NSE gamma Monoclonal Antibody (ENO2/1375).
Mouse Anti-NSE gamma Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439932

Western Blot (WB) of anti-NSE gamma antibody

Western Blot of Y79, HeLa and HepG2 Cell Lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).
Mouse Anti-NSE gamma Monoclonal Antibody, Western Blot (WB) – Cat# MBS439932

Immunohistochemistry (IHC) of anti-NSE gamma antibody

Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1375).
Mouse Anti-NSE gamma Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439932

Immunohistochemistry (IHC) of anti-NSE gamma antibody

Formalin-fixed, paraffin-embedded Rat Heart stained with Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1375).
Mouse Anti-NSE gamma Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439932

Product References and Citations for anti-NSE gamma antibody

Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.

Other Notes

Small volumes of anti-NSE gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NSE gamma. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
UniProt Protein Name
Gamma-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
NCBI Protein Information
gamma-enolase

NCBI Summary for NSE gamma

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

UniProt Comments for NSE gamma

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NSE gamma antibody

 Reference Product  PubMed Publications
 HK1 antibody  >2 publications with NSE gamma and HK1
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