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ETS1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439946

MBS439946 is an antibody targeting ETS1. Also known as ETS1 (Marker of Tumor Metastasis). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ETS1, Monoclonal Antibody
Full Product Name
ETS1 (Marker of Tumor Metastasis)
Product Gene Name
Product Synonym Names
Avian erythroblastosis virus E26 (v ets) oncogene homolog 1; ETS1; EWSR2; p54; v-ets erythroblastosis virus E26 oncogene homolog 1
Clonality
Monoclonal
Isotype
IgG
Clone Number
ETS1/1282
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
ETS1 proto-oncogene is an important transcription factor that plays a role in cell proliferation and differentiation. ETS1 is related to the growth of carcinoma cells by its regulation of the transcription of matrix metalloproteinases and urokinase-type plasminogen activator. The processes of tumor invasion and metastasis depend on the increased proteolytic activity of the invading tumor cells that may involve matrix metalloproteinases, cathepsins B and D and plasminogen activator in the metastatic cascade. ETS1 is preferentially expressed in lymphoid cells, where it is essential for the maintenance of the normal pool of resting T and B cells. ETS1 expression level and distribution are differentially controlled in resting, activated and apoptotic lymphocytes.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
54kDa
3D Structure
ModBase 3D Structure for P14921
Immunogen
Recombinant human ETS1 fragment (aa137-230)
Cellular Localization
Nuclear
Hu-chromosome Location
11q23.3
Positive Control
HepG2 and HeLa cells. Tonsil, Skin, Pancreas, Kidney and Lymphoma.
Applications Tested/Suitable for anti-ETS1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ETS1 antibody
FC/FACS: 0.5-1ug/million cells in 0.1ml
IF: 1-2ug/ml
WB: 1-2ug/ml for 60 minutes at RT
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-ETS1 antibody

Formalin-fixed, paraffin-embedded human Spleen stained with ETS1 Monoclonal Antibody (ETS1/1282).
Mouse Anti-ETS1 Monoclonal Antibody – Cat# MBS439946

Product References and Citations for anti-ETS1 antibody

Gaspar Kitange, Keishi Tsunoda, Takeo Anda, Shunsuke Nakamura, Akio Yasunaga, Shinji Naito, Shobu Shibata. Immunohistochemical expression of Ets-1 transcription factor and the urokinase-type plasminogen activator is correlated with the malignant and invasive potential in meningiomas. Cancer 89 (11), 2292-2300, 2000.

Other Notes

Small volumes of anti-ETS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ETS1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein C-ets-1 isoform 1
NCBI Official Synonym Full Names
ETS proto-oncogene 1, transcription factor
NCBI Official Symbol
ETS1
NCBI Official Synonym Symbols
p54; ETS-1; EWSR2; c-ets-1
UniProt Protein Name
Protein C-ets-1
UniProt Synonym Protein Names
p54
Protein Family
UniProt Gene Name
ETS1
UniProt Synonym Gene Names
EWSR2
NCBI Protein Information
protein C-ets-1

NCBI Summary for ETS1

This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
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UniProt Comments for ETS1

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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