Mouse Anti-GJB1 Monoclonal Antibody – Cat# MBS439991

Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A Monoclonal Antibody (MLANA/788).

Connexin 32 (GJB1) Antibody – Mouse Monoclonal

Name: GJB1 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439991
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439991

MBS439991 is an antibody targeting GJB1. Also known as Connexin 32 (Gap Junction Protein). Reactive in Human, Mouse, Rat. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Connexin 32 (GJB1), Monoclonal Antibody

Full Product Name

Connexin 32 (Gap Junction Protein)

Product Gene Name

anti-GJB1 antibody

Product Synonym Names

Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1

Clonality

Monoclonal

Isotype

IgG

Clone Number

M12.13

Host

Mouse

Species Reactivity

Human, Mouse, Rat. Others not known.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

27-32kDa

3D Structure

ModBase 3D Structure for P08034

Immunogen

Rat junctional complexes

Cellular Localization

Cytoplasmic and Cell Surface

Hu-chromosome Location

Xq13.1

Positive Control

MCF-7 Cells. Liver, Kidney, Stomach or Tonsil

Applications Tested/Suitable for anti-GJB1 antibody

Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Application Notes for anti-GJB1 antibody

IF: 1-2ug/ml
FC/FACS: 0.5-1ug/million cells
WB: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT.

Product References and Citations for anti-GJB1 antibody

D. A. Goodenough et. al. Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions. Journal of Cell Biology, 107(5), 1817-1824 (1988).

Other Notes

Small volumes of anti-GJB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GJB1. It may not necessarily be applicable to this product.

NCBI GI #

4504005

NCBI GeneID

2705

NCBI Accession #

NP_000157.1

UniProt Primary Accession #

P08034

UniProt Secondary Accession #

Q5U0S4; B2R8R2; D3DVV2

UniProt Related Accession #

P08034

GenBank Nucleotide #

NM_000166.5

NCBI Official Full Name

gap junction beta-1 protein

NCBI Official Synonym Full Names

gap junction protein beta 1

NCBI Official Symbol

GJB1

NCBI Official Synonym Symbols

CMTX; CX32; CMTX1

UniProt Protein Name

Gap junction beta-1 protein

UniProt Synonym Protein Names

Connexin-32; Cx32; GAP junction 28 kDa liver protein

Protein Family

Gap junction beta-1 protein

UniProt Gene Name

GJB1

UniProt Synonym Gene Names

CX32; Cx32

NCBI Protein Information

gap junction beta-1 protein

NCBI Summary for GJB1

This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2008]

UniProt Comments for GJB1

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Research Articles on GJB1

1. Results show that the GJB1 (connexin 32; Cx32) mutants R75P, R75Q and R75W display variable structural conformation and dynamic behavior compared to the native protein.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GJB1 antibody

Reference Product	PubMed Publications
GJB2 antibody	>12 publications with GJB1 and GJB2

Pathways associated with anti-GJB1 antibody

Products by Pathway	Pathway Diagram
Calcium Regulation In The Cardiac Cell Pathway antibodies	Calcium Regulation In The Cardiac Cell Pathway Diagram
Gap Junction Assembly Pathway antibodies	Gap Junction Assembly Pathway Diagram
Gap Junction Trafficking Pathway antibodies	Gap Junction Trafficking Pathway Diagram
Gap Junction Trafficking And Regulation Pathway antibodies	Gap Junction Trafficking And Regulation Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Oligomerization Of Connexins Into Connexons Pathway antibodies	Oligomerization Of Connexins Into Connexons Pathway Diagram
Transport Of Connexins Along The Secretory Pathway antibodies	Transport Of Connexins Along The Secretory Pathway Diagram

Diseases associated with anti-GJB1 antibody

Disease Name	Pubmed Publications
Neuromuscular Diseases Antibodies	>109 publications with GJB1 and Neuromuscular Diseases
Muscle Weakness Antibodies	>12 publications with GJB1 and Muscle Weakness
Brain Diseases Antibodies	>8 publications with GJB1 and Brain Diseases
Nerve Degeneration Antibodies	>6 publications with GJB1 and Nerve Degeneration
Disease Models, Animal Antibodies	>4 publications with GJB1 and Disease Models, Animal
Neoplasms Antibodies	>3 publications with GJB1 and Neoplasms
Pain Antibodies	>3 publications with GJB1 and Pain
Eye Diseases Antibodies	>2 publications with GJB1 and Eye Diseases
Charcot-Marie-Tooth disease, X-linked, 1 Antibodies	>2 publications with GJB1 and Charcot-Marie-Tooth disease, X-linked, 1
Reflex, Abnormal Antibodies	>1 publications with GJB1 and Reflex, Abnormal

Organs/Tissues associated with anti-GJB1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>16 publications with GJB1 and Brain
Liver Antibodies	>4 publications with GJB1 and Liver
Heart Antibodies	>2 publications with GJB1 and Heart
Skin Antibodies	>2 publications with GJB1 and Skin
Embryonic Tissue Antibodies	>1 publications with GJB1 and Embryonic Tissue
Mammary Gland Antibodies	>1 publications with GJB1 and Mammary Gland
Testis Antibodies	>1 publications with GJB1 and Testis
Kidney Antibodies	>1 publications with GJB1 and Kidney
Pancreas Antibodies	>1 publications with GJB1 and Pancreas