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HIS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS440057

The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a… More details

$325 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIS tag, Polyclonal Antibody
Full Product Name
HIS tag (H20) Antibody
Product Gene Name
anti-HIS antibody
Clonality
Polyclonal
Host
Rabbit
Form/Format
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Concentration
0.200 mg/ml (lot specific)
Specificity
Detects proteins with the polyhistidine Tag
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Antibody/Peptide Pairs
HIS peptide (MBS443057) is used for blocking the activity of HIS antibody (MBS440057)
Origin
His (H20) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide containing the 6x histidine tag.
Immunogen
Synthetic a peptide containing the 6x histidine tag.
Applications Tested/Suitable for anti-HIS antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-HIS antibody
Western blot analysis of polyhistidine tagged recombinant HspBP1 at 0.1 ug (A) and 0.01 ug (B).

Western Blot (WB) of anti-HIS antibody

Rabbit Anti-HIS Polyclonal Antibody – Cat# MBS440057

Product References and Citations for anti-HIS antibody

1.) Wu B, Wilmouth RC. 2008. Proteomics analysis of immunoprecipitated proteins associated with the oncogenic kinase cot. Mol Cells. Feb 29;25(1):43-9.

Other Notes

Small volumes of anti-HIS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIS purchase

MBS440057 is an antibody targeting HIS. Also known as HIS tag (H20) Antibody. Host: Rabbit.
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