CDC2 p34 Antibody – Mouse Monoclonal

Cyclin dependent kinases are key regulators of the progression of the cell cycle. Early in the cell cycle Cdk2, Cdk4, Cdk6 and their associated cyclins regulate the G1 to S phase transition (1, 2). Cdk2 plays a key role in the G1/S and S/G2 transitions through its associations with cyclin D1, cyclin D2, cyclin D3, cyclin E and cyclin A. Cdk4 also forms complexes with the D type cyclins, and is thought to regulate cell growth through the G1 phase of the cell cycle (3-6). The late stages of the cell cycle are regulated by another cyclin dependent kinase, Cdc2 p34. This kinase exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition (7,8).

1. Ekholm SV, Reed SI. 2000. Regulation of G(1) cyclin-dependent kinases in the mammalian cell cycle. Curr Opin Cell Biology 12(6): 676-6842. Morisaki H, Ando A, Nagata Y, Pereira-Smith O, Smith JR, Ikeda K, Nakanishi M. 1999. Complex mechanisms underlying impaired activation of Cdk4 and Cdk2 in replicative senescence: roles of p16, p21, and cyclin D1. Exp Cell Research 253(2): 503-510.3. Tong W, Pollard JW. 1999. Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biology 19(3): 2251-64.4. Sweeney KJ, Sarcevic B, Sutherland RL, Musgrove EA. 1997. Cyclin D2 activates Cdk2 in preference to Cdk4 in human breast eptithelial cells. Oncogene 14(11): 1329-1340.5. Chu CY, Lim RW. 2000. Involvement of p27(kip1) and cyclin D3 in the regulation of cdk2 activity during skeletal muscle differentiation. Biochimica Biophysica Acta 1497(2): 175-185.6. Kishimoto T, Okumura E. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. 1997. Prog Cell Cycle Res 3:241-249.7. Morla AO, Draetta G, Beach D, Wang JY. 1989. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. Cell 58(1): 193-203.8. O?Connor PM, Ferris DK, Pagano M, Draetta G, Pines J, Hunter T, Longo DL, Kohn KW. 1993. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently. J Biological Chemistry 268(11): 8298-8308.

Small volumes of anti-Cdc2 p34 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS440092 is an antibody targeting Cdc2 p34. Also known as Cdc2 p34 (17) Antibody. Reactive in Mouse, human, Xenopus. Host: Mouse.