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Chondroitin Sulphate Neoepitope Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS442014

Chondroitin sulfate monoclonal antibody (mouse, clone 1B5) to detect the zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.Overview: Recognizes delta unsaturated disaccharide of unsulfated chondroitin generated by… More details

$670 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Chondroitin Sulphate Neoepitope, Monoclonal Antibody
Full Product Name
Monocloncal Antibody to Chondroitin Sulphate Neoepitope Antibody (clone 1B5)
Product Synonym Names
Chondroitin Sulphate Neoepitope Antibody
Clonality
Monoclonal
Isotype
IgG1K
Clone Number
1B5
Host
Mouse
Form/Format
Clear liquid
Purity/Purification
Protein A
Concentration
0.1 mg/mL (lot specific)
Specificity
This antibody recognizes zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.
Preparation and Storage
Store at -20 degree C. Avoid repeating freezing and thawing. Aliquoting is recommended.
Immunogen
Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABC digestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).
Buffer
PBS, no preservatives
Technical Notes
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
Applications Tested/Suitable for anti-Chondroitin Sulphate Neoepitope antibody
Western Blot (WB), Immunohistochemistry (IHC)
Western Blot: use at dilution of 1/100.; Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin embedded sections, alcohol-fixed frozen sections or un-fixed snap-frozen sections.

Other Notes

Small volumes of anti-Chondroitin Sulphate Neoepitope antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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MBS442014 is an antibody targeting Chondroitin Sulphate Neoepitope. Also known as Monocloncal Antibody to Chondroitin Sulphate Neoepitope Antibody (clone 1B5). Host: Mouse.
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