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Thyrotropin Releasing Hormone (TRH) ELISA Kit for Chicken

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS044339

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies… More details

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Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thyrotropin Releasing Hormone (TRH), ELISA Kit
Full Product Name
Chicken Thyrotropin Releasing Hormone ELISA Kit
Product Gene Name
TRH elisa kit
Species Reactivity
Chicken
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Detection Range
0.625 ?IU/ml - 20 ?IU/ml.
Sensitivity
0.1 ?IU/ml.
Specificity
No significant cross-reactivity or interference between Chicken TRH and analogues was observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
27,404 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (129693114..129696778). Location: 3q13.3-q21
OMIM
613879
3D Structure
ModBase 3D Structure for P20396
Intended Uses
ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate TRH concentrations in Chicken serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications

Typical Testing Data/Standard Curve (for reference only) of TRH elisa kit

Chicken TRH ELISA Kit – Cat# MBS044339

Product References and Citations for TRH elisa kit

Blockade of neurokinin-1 receptors in the ventral respiratory column does not affect breathing but alters neurochemical release. Clarissa Muere, Suzanne Neumueller, Samantha Olesiak, Justin Miller, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster (2015)

Other Notes

Small volumes of TRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRH purchase

MBS044339 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thyrotropin Releasing Hormone (TRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRH. The ELISA analytical biochemical technique of the MBS044339 kit is based on TRH antibody-TRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TRH. It may not necessarily be applicable to this product.

NCBI GI #
6005920
NCBI GeneID
7200
NCBI Accession #
NP_009048.1
UniProt Primary Accession #
P20396
UniProt Secondary Accession #
Q2TB83; B2R8R1
UniProt Related Accession #
P20396
GenBank Nucleotide #
NM_007117.4
NCBI Official Full Name
thyrotropin releasing hormone preproprotein
NCBI Official Synonym Full Names
thyrotropin-releasing hormone
NCBI Official Symbol
TRH
NCBI Official Synonym Symbols
TRF
UniProt Protein Name
Pro-thyrotropin-releasing hormone
UniProt Synonym Protein Names
ProthyroliberinCleaved into the following chain:Thyrotropin-releasing hormone; TRH; Alternative name(s):; Protirelin; TSH-releasing factor; Thyroliberin; Thyrotropin-releasing factor
Protein Family
Pro-thyrotropin-releasing hormone
UniProt Gene Name
TRH
UniProt Synonym Gene Names
Pro-TRH; TRH; TRF
UniProt Entry Name
TRH_HUMAN
NCBI Protein Information
thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor

NCBI Summary for TRH

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]
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UniProt Comments for TRH

TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q13.3-q21

Cellular Component: plasma membrane; extracellular region; nucleus; secretory granule

Molecular Function: neuropeptide hormone activity; thyrotropin-releasing hormone activity

Biological Process: hormone-mediated signaling; negative regulation of glutamate secretion; eating behavior; positive regulation of insulin secretion; histamine metabolic process; signal transduction; adult walking behavior; response to ethanol; cell-cell signaling; response to hypoxia; response to glucose stimulus; response to cold; response to corticosterone stimulus; positive regulation of gamma-aminobutyric acid secretion

Disease: Thyrotropin-releasing Hormone Deficiency
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Research Articles on TRH

1. Cpe mutations in mouse may affect processing of prothyrotropin releasing hormone into thyrotropin releasing hormone.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TRH elisa kit

 Reference Product  PubMed Publications
 PRL elisa kit  >1751 publications with TRH and PRL
 GHRH elisa kit  >246 publications with TRH and GHRH
 CCK elisa kit  >62 publications with TRH and CCK
 TRHR elisa kit  >47 publications with TRH and TRHR
 NTS elisa kit  >14 publications with TRH and NTS
 GRP elisa kit  >8 publications with TRH and GRP
 GCG elisa kit  >2 publications with TRH and GCG
 GNRH1 elisa kit  >1 publications with TRH and GNRH1

Pathways associated with TRH elisa kit

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with TRH elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1987 publications with TRH and Nervous System Diseases
 Brain Diseases Antibodies  >1675 publications with TRH and Brain Diseases
 THYROTROPIN-RELEASING HORMONE DEFICIENCY Antibodies  >282 publications with TRH and THYROTROPIN-RELEASING HORMONE DEFICIENCY
 Disease Models, Animal Antibodies  >130 publications with TRH and Disease Models, Animal
 Drug Toxicity Antibodies  >108 publications with TRH and Drug Toxicity
 Bipolar Disorder Antibodies  >102 publications with TRH and Bipolar Disorder
 Seizures Antibodies  >94 publications with TRH and Seizures
 Hypothermia Antibodies  >91 publications with TRH and Hypothermia
 Hypertension Antibodies  >87 publications with TRH and Hypertension
 Pain Antibodies  >83 publications with TRH and Pain

Organs/Tissues associated with TRH elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4618 publications with TRH and Brain
 Ovary Antibodies  >123 publications with TRH and Ovary
 Lung Antibodies  >111 publications with TRH and Lung
 Eye Antibodies  >109 publications with TRH and Eye
 Pineal Gland Antibodies  >48 publications with TRH and Pineal Gland
 Bone Marrow Antibodies  >12 publications with TRH and Bone Marrow
 Cervix Antibodies  >3 publications with TRH and Cervix
 Uncharacterized Tissue Antibodies  >1 publications with TRH and Uncharacterized Tissue
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