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CCL28 Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS444009

Mucosae-associated epithelial chemokine (MEC/CCL28) is secreted by epithelial cells of gastrointestinal and nonintestinal mucosal cells, and regulates chemotaxis of cells expressing surface receptors CCR3 and CCR10. It shows broad-spectrum antibacterial… More details

$175 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CCL28, Active Protein
Full Product Name
Human CCL28 (MEC)
Product Gene Name
Product Synonym Names
CCL28 (MEC)
Sequence
ILPIASSCCT EVSHHISRRL LERVNMCRIQ RADGDCDLAA VILHVKRRRI CVSPHNHTVK QWMKVQAAKK NGKGNVCHRK KHHGKRNSNR AHQGKHETYG HKTPY
Host
E Coli derived
Form/Format
Lyophilized
Purity/Purification
> 97% by SDS PAGE
Preparation and Storage
Avoid repeated freeze-thaw cycles
12 months from date of receipt, -20 to -70 degree C as supplied.
1 month, 2 to 8 degree C under sterile conditions after reconstitution.
3 months, -20 to -70 degree C under sterile conditions after reconstitution.
Matching Pairs
Unconjugated Antibody: CCL28 (MBS444009)
Biotin Conjugated Antibody: CCL28 (MBS444015)
Molecular Weight
Actual Molecular Mass: Mass Spec data confirmed expected mass of 12.031kDa
Predicted Molecular Mass: 12.031 kDa
Sequence Positions
23-127
OMIM
605240
3D Structure
ModBase 3D Structure for Q9NRJ3
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Reconstitution
Spin sample prior to reconstitution. Recommended at 100mug/mL in sterile water
Carrier Protein
None
Activity
EC50 = 38nM determined by migration assay with cells expressing recombinant CCR10
Extinction Coefficient
8,970 M-1 cm-1
Endotoxin Level
<0.01 EU per 1mug of the protein by the LAL method
Modification
None
UNSPSC Code
12352202

Testing Data of CCL28 active protein

Human CCL28 Active Protein – Cat# MBS444009

Product References and Citations for CCL28 active protein

1. "A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues" Pan et al. J. Immunol. 165: 2943-2949 (2000)
2. Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2). Wang et al. J. Biol. Chem. 275: 22313-22323 (2000)
3. "The Mucosae-Associated Epithelial Chemokine (MEC/CCL28) Modulates Immunity in HIV Infection" Castelletti E. at al. PLoS ONE 2:e969 (2007)

Other Notes

Small volumes of CCL28 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL28 purchase

MBS444009 is an active protein targeting CCL28. Also known as Human CCL28 (MEC). Host: E Coli derived.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL28. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
C-C motif chemokine 28 isoform a
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 28
NCBI Official Symbol
CCL28
NCBI Official Synonym Symbols
MEC; CCK1; SCYA28
UniProt Synonym Protein Names
Mucosae-associated epithelial chemokine; MEC; Protein CCK1; Small-inducible cytokine A28
Protein Family
UniProt Gene Name
CCL28
UniProt Synonym Gene Names
SCYA28; MEC
UniProt Entry Name
CCL28_HUMAN
NCBI Protein Information
C-C motif chemokine 28; CC chemokine CCL28; chemokine (C-C motif) ligand 28 splice variant chi; mucosae-associated epithelial chemokine; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys), member 28; small-inducible cytokine A28

NCBI Summary for CCL28

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
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UniProt Comments for CCL28

CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: elevation of cytosolic calcium ion concentration; immune response; positive regulation of cell-matrix adhesion; chemotaxis; response to nutrient
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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