Human CCL3 Active Protein – Cat# MBS444016

Migration Assay: Cells expressing recombinant CCR1 were assayed for migration through a transwell filter at various concentrations of MIP-1alpha. Responses are expressed as the % of total input cells.

CCL3 Human Active Protein

Name: CCL3 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS444016
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS444016

Macrophage inflammatory proteins 1-alpha (MIP-1alpha/CCL3) binds to cell surface receptors CCR1 and CCR5. It is a proinflammatory chemokine, leading to chemotaxis and activation of immune cells. It also inhibits proliferation of hematopoietic stem cells.… More details

$220 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CCL3, Active Protein

Full Product Name

Human CCL3 (MIP-1alpha)

Product Gene Name

CCL3 active protein

Product Synonym Names

CCL3 (MIP-1alpha) Biotin

Sequence

SLAADTPTAC CFSYTSRQIP QNFIADYFET SSQCSKPGVI FLTKRSRQVC ADPSEEWV QKYVSDLELS A

Host

E Coli derived

Form/Format

Lyophilized

Purity/Purification

> 97% by SDS PAGE

Preparation and Storage

Avoid repeated freeze-thaw cycles
12 months from date of receipt, -20 to -70 degree C as supplied.
1 month, 2 to 8 degree C under sterile conditions after reconstitution.
3 months, -20 to -70 degree C under sterile conditions after reconstitution.

Matching Pairs

Unconjugated Antibody: CCL3 (MBS444002)
Biotin Conjugated Antibody: CCL3 (MBS444016)

Molecular Weight

Actual Molecular Mass: Mass spec data confirmed the expected mass of 7.716 kDa
Predicted Molecular Mass: 7.716 kDa

Sequence Positions

24-92

OMIM

182283

3D Structure

ModBase 3D Structure for P10147

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Reconstitution

Spin sample prior to reconstitution. Recommended at 100mug/mL in sterile water

Carrier Protein

None

Activity

EC50 = 0.1-0.3 nM determined by migration assay with cells expressing recombinant CCR5

Extinction Coefficient

10,010 M-1 cm-1

Endotoxin Level

<0.01 EU per 1mug of the protein by the LAL method

Modification

None

UNSPSC Code

12352202

Product References and Citations for CCL3 active protein

1. "Macrophage inflammatory protein-1" Menten P., Wuyts A., Van Damme J. Cytokine Growth Factor Rev. 13:455-481(2002)
2. "Identification of RANTES, MIP-1, and MIP-1[1] as the major HIV-suppressive factors produced by CD8+T cells" Cocci F., De Vico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P. Science 270:1811-1815 (1995)
3. "Identification and characterization of an inhibitor of haemopoietic stem cell proliferation" Graham G.J., Wright E.G., Hewick R., Wolpe S.D., Wilkie N.M., Donaldson D., Lorimore S., Pragnell I.B. Nature 344:442-444 (1990)
4. "Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors" Zipfel P.F., Balke J., Irving S.G., Kelly K., Siebenlist U. J Immunol 142:1582-1590 (1989)

Other Notes

Small volumes of CCL3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL3 purchase

MBS444016 is an active protein targeting CCL3. Also known as Human CCL3 (MIP-1alpha). Host: E Coli derived.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL3. It may not necessarily be applicable to this product.

NCBI GI #

4506843

NCBI GeneID

6348

NCBI Accession #

NP_002974.1

UniProt Primary Accession #

P10147

UniProt Related Accession #

P10147

GenBank Nucleotide #

NM_002983.2

NCBI Official Full Name

C-C motif chemokine 3

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 3

NCBI Official Symbol

CCL3

NCBI Official Synonym Symbols

MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha

UniProt Protein Name

C-C motif chemokine 3

UniProt Synonym Protein Names

G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha

Protein Family

C-C motif chemokine

UniProt Gene Name

CCL3

UniProt Synonym Gene Names

MIP-1-alpha

UniProt Entry Name

CCL3_HUMAN

NCBI Protein Information

C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; PAT 464.1; SIS-beta; macrophage inflammatory protein 1-alpha; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein

NCBI Summary for CCL3

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

UniProt Comments for CCL3

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; chemoattractant activity; phospholipase activator activity; protein kinase activity; CCR5 chemokine receptor binding

Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; negative regulation of bone mineralization; monocyte chemotaxis; regulation of cell shape; positive chemotaxis; cell-cell signaling; positive regulation of neuron apoptosis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cell activation; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

Research Articles on CCL3

1. In COPD patients, CCL3 is upregulated in sputum and may facilitate recruitment of macrophages into airways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CCL3 active protein

Reference Product	PubMed Publications
CCL4 active protein	>1638 publications with CCL3 and CCL4
CCR5 active protein	>513 publications with CCL3 and CCR5
CCR1 active protein	>286 publications with CCL3 and CCR1
CCR2 active protein	>141 publications with CCL3 and CCR2
CCR3 active protein	>69 publications with CCL3 and CCR3
CCR4 active protein	>47 publications with CCL3 and CCR4
IL12B active protein	>5 publications with CCL3 and IL12B
IDE active protein	>5 publications with CCL3 and IDE
CXCR5 active protein	>4 publications with CCL3 and CXCR5
CCBP2 active protein	>4 publications with CCL3 and CCBP2

Pathways associated with CCL3 active protein

Products by Pathway	Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
IL12-mediated Signaling Events Pathway antibodies	IL12-mediated Signaling Events Pathway Diagram

Diseases associated with CCL3 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>922 publications with CCL3 and Inflammation
Necrosis Antibodies	>522 publications with CCL3 and Necrosis
Disease Models, Animal Antibodies	>321 publications with CCL3 and Disease Models, Animal
Lung Diseases Antibodies	>246 publications with CCL3 and Lung Diseases
HIV Infections Antibodies	>188 publications with CCL3 and HIV Infections
Cardiovascular Diseases Antibodies	>177 publications with CCL3 and Cardiovascular Diseases
Hypersensitivity Antibodies	>106 publications with CCL3 and Hypersensitivity
Liver Diseases Antibodies	>99 publications with CCL3 and Liver Diseases
Fibrosis Antibodies	>84 publications with CCL3 and Fibrosis
Pneumonia Antibodies	>73 publications with CCL3 and Pneumonia

Organs/Tissues associated with CCL3 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1582 publications with CCL3 and Blood
Lung Antibodies	>338 publications with CCL3 and Lung
Brain Antibodies	>262 publications with CCL3 and Brain
Liver Antibodies	>222 publications with CCL3 and Liver
Kidney Antibodies	>86 publications with CCL3 and Kidney
Heart Antibodies	>79 publications with CCL3 and Heart
Intestine Antibodies	>56 publications with CCL3 and Intestine
Eye Antibodies	>51 publications with CCL3 and Eye
Uterus Antibodies	>19 publications with CCL3 and Uterus
Placenta Antibodies	>18 publications with CCL3 and Placenta