Human CCL4 Active Protein – Cat# MBS444017

CCL4 Human Active Protein

Name: CCL4 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS444017
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS444017

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian… More details

$220 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CCL4, Active Protein

Full Product Name

Human CCL4 (MIP-1beta)

Product Gene Name

CCL4 active protein

Product Synonym Names

CCL4 (MIP-1beta) Biotin

Sequence

APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSES WVQEYVYDLE LN

Host

E Coli derived

Form/Format

Lyophilized

Purity/Purification

> 97% by SDS PAGE

Preparation and Storage

Avoid repeated freeze-thaw cycles
12 months from date of receipt, -20 to -70 degree C as supplied.
1 month, 2 to 8 degree C under sterile conditions after reconstitution.
3 months, -20 to -70 degree C under sterile conditions after reconstitution.

Matching Pairs

Unconjugated Antibody: CCL4 (MBS444021)
Biotin Conjugated Antibody: CCL4 (MBS444017)

Molecular Weight

Actual Molecular Mass: Mass spec data confirmed the expected mass of 7.818 kDa
Predicted Molecular Mass: 7.818 kDa

Sequence Positions

24-92

OMIM

182284

3D Structure

ModBase 3D Structure for P13236

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Reconstitution

Spin sample prior to reconstitution. Recommended at 100mug/mL in sterile water.

Carrier Protein

None

Activity

EC50 = 0.23nM determined by Migration Assay with cells expressing recombinant CCR5

Extinction Coefficient

12570 M-1 cm-1

Endotoxin Level

<0.01 EU per 1mug of the protein by the LAL method

Modification

None

UNSPSC Code

12352202

Product References and Citations for CCL4 active protein

1. "Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells." Cocchi F., DeVico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P. Science 270:1811-1815 (1995)
2. "The assignment of chemokine-chemokine receptor pairs: TARC and MIP-1 beta are not ligands for human CC-chemokine receptor 8." Garlisi C.G., Xiao H., Tian F., Hedrick J.A., Billah M.M., Egan R.W., Umland S.P. Eur. J. Immunol. 29:3210-3215 (1999)
3. "Natural truncation of the chemokine MIP-1beta/CCL4 affects receptor specificity but not anti-HIV-1 activity." Guan E., Wang J., Roderiquez G., Norcross M.A. J. Biol. Chem. 277:32348-32352 (2002)

Other Notes

Small volumes of CCL4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL4 purchase

MBS444017 is an active protein targeting CCL4. Also known as Human CCL4 (MIP-1beta). Host: E Coli derived.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL4. It may not necessarily be applicable to this product.

NCBI GI #

4506845

NCBI GeneID

6351

NCBI Accession #

NP_002975.1

UniProt Primary Accession #

P13236

UniProt Secondary Accession #

P22617; Q13704; Q3SXL8; Q6FGI8

UniProt Related Accession #

P13236

GenBank Nucleotide #

NM_002984.3

NCBI Official Full Name

C-C motif chemokine 4

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 4

NCBI Official Symbol

CCL4

NCBI Official Synonym Symbols

ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta

UniProt Protein Name

C-C motif chemokine 4

UniProt Synonym Protein Names

G-26 T-lymphocyte-secreted protein; HC21; Lymphocyte activation gene 1 protein; LAG-1

Protein Family

C-C motif chemokine

UniProt Gene Name

CCL4

UniProt Synonym Gene Names

LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2

UniProt Entry Name

CCL4_HUMAN

NCBI Protein Information

C-C motif chemokine 4; G-26 T-lymphocyte-secreted protein; MIP-1-beta(1-69); PAT 744; SIS-gamma; T-cell activation protein 2; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; secreted protein G-26; small inducible cytokine A4 (homologous to mouse Mip-1b)

NCBI Summary for CCL4

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

UniProt Comments for CCL4

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: cell-cell signaling; response to toxin; response to virus; establishment and/or maintenance of cell polarity; immune response; positive regulation of calcium-mediated signaling; signal transduction; positive regulation of calcium ion transport; inflammatory response; cell adhesion; cell motility

Research Articles on CCL4

1. the secretion of IL-1alpha, IL-1beta, IL-12b, and CCL4 occurs during gelatinase degranulation, a process controlled by STX3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CCL4 active protein

Reference Product	PubMed Publications
CCL3 active protein	>1638 publications with CCL4 and CCL3
CCR5 active protein	>601 publications with CCL4 and CCR5
CCR1 active protein	>200 publications with CCL4 and CCR1
CCR2 active protein	>111 publications with CCL4 and CCR2
CXCR3 active protein	>76 publications with CCL4 and CXCR3
CCR3 active protein	>73 publications with CCL4 and CCR3
CCR7 active protein	>53 publications with CCL4 and CCR7
CCL17 active protein	>50 publications with CCL4 and CCL17
IDE active protein	>5 publications with CCL4 and IDE
CCBP2 active protein	>1 publications with CCL4 and CCBP2

Pathways associated with CCL4 active protein

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
IL12-mediated Signaling Events Pathway antibodies	IL12-mediated Signaling Events Pathway Diagram

Diseases associated with CCL4 active protein

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>698 publications with CCL4 and Immune System Diseases
Disease Progression Antibodies	>241 publications with CCL4 and Disease Progression
Protein Deficiency Antibodies	>162 publications with CCL4 and Protein Deficiency
Bone Diseases Antibodies	>108 publications with CCL4 and Bone Diseases
Hypertension, Portal Antibodies	>93 publications with CCL4 and Hypertension, Portal
Disease Susceptibility Antibodies	>78 publications with CCL4 and Disease Susceptibility
Eye Diseases Antibodies	>74 publications with CCL4 and Eye Diseases
Pneumonia Antibodies	>69 publications with CCL4 and Pneumonia
Ischemia Antibodies	>62 publications with CCL4 and Ischemia
Diabetes Mellitus Antibodies	>52 publications with CCL4 and Diabetes Mellitus

Organs/Tissues associated with CCL4 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>4488 publications with CCL4 and Liver
Blood Antibodies	>3319 publications with CCL4 and Blood
Lung Antibodies	>409 publications with CCL4 and Lung
Bone Marrow Antibodies	>303 publications with CCL4 and Bone Marrow
Kidney Antibodies	>293 publications with CCL4 and Kidney
Intestine Antibodies	>119 publications with CCL4 and Intestine
Lymph Antibodies	>112 publications with CCL4 and Lymph
Parathyroid Antibodies	>14 publications with CCL4 and Parathyroid
Prostate Antibodies	>13 publications with CCL4 and Prostate
Adrenal Gland Antibodies	>7 publications with CCL4 and Adrenal Gland