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RFP Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS448122
31 units are in stock for immediate shipment. Please contact us for more details.

Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. More details

$405 / 0.3 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RFP, Polyclonal Antibody
Full Product Name
RFP Polyclonal Antibody
Product Gene Name
Product Synonym Names
Cherry fluorescent protein; dsRed; red fluorescent protein; tdTomato antibody
Clonality
Polyclonal
Host
Goat
Species Reactivity
Reacts with Transfected cells proteins.
Form/Format
Polyclonal antibody supplied as a 100 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Purity/Purification
This antibody is epitope-affinity purified from goat antiserum.
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Preparation and Storage
For continuous use, store at 2-8 degrees C for one-two days. For extended storage, store in -20 degrees C freezer. Working dilution samples should be discarded if not used within 12 hours.
Immunogen
Purified recombinant RFP peptide produced in E Coli.
Handling
The antibody solution should be gently mixed before use.
Applications Tested/Suitable for anti-RFP antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry Formalin/Paraffin (IHC-F/IHC-P)
Application Notes for anti-RFP antibody
Western blot: 1:500-1:2,000
Immunofluorescence: 1:50-1:500
Immunohistochemistry (paraffin): 1:50-1:500
Immunohistochemistry (frozen): 1:50-1:500

Reactivity Data of anti-RFP antibody

+++ excellent, ++ good, + poor, ND - not determined
Goat Anti-RFP Polyclonal Antibody – Cat# MBS448122

Immunofluorescence (IF) of anti-RFP antibody

Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing RFP in neurons (DyGlutRFP) using 1st Ab anti-RFP at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight488 at 1/500;
Goat Anti-RFP Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS448122

Immunofluorescence (IF) of anti-RFP antibody

Immunofluorescence - anti-RFP Ab in 293HEK cells transfected with RFP at 1/50 dilution; cells were fixed with 4% of PFA;
Goat Anti-RFP Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS448122

Western Blot (WB) of anti-RFP antibody

Anti-RFP Ab at 1/2,000 dilution; 293HEK cells transduced with RFP Ad; lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Goat Anti-RFP Polyclonal Antibody, Western Blot (WB) – Cat# MBS448122

Immunohistochemistry (IHC) of anti-RFP antibody

Immunogold labeling of epithelium cells, in vivo injected with RFP expressing vector;
Goat Anti-RFP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS448122

Other Notes

Small volumes of anti-RFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFP purchase

MBS448122 is an antibody targeting RFP. Also known as RFP Polyclonal Antibody. Reactive in Reacts with Transfected cells proteins.. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for RFP. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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