Human EG-VEGF ELISA Kit – Cat# MBS450441

Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit for Human

Name: Human EG-VEGF ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS450441
Price: 355 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS450441

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EG-VEGF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Principle of the Assay: The microtiter plate… More details

$355 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF), ELISA Kit

Full Product Name

Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

Product Gene Name

EG-VEGF elisa kit

Product Synonym Names

GRO1; CXCL1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1;

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range

15.625-1000pg/mL

Sensitivity

7.2pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of EG-VEGF. No significant cross-reactivity or interference between EG-VEGF and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EG-VEGF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EG-VEGF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

11,715 Da

OMIM

AF333024 mRNA

3D Structure

ModBase 3D Structure for P58294

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of EG-VEGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EG-VEGF purchase

MBS450441 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EG-VEGF. The ELISA analytical biochemical technique of the MBS450441 kit is based on EG-VEGF antibody-EG-VEGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EG-VEGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EG-VEGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EG-VEGF. It may not necessarily be applicable to this product.

NCBI GI #

14165282

NCBI GeneID

84432

NCBI Accession #

NP_115790.1

UniProt Primary Accession #

P58294

UniProt Secondary Accession #

Q5VWD4; Q8TC69

UniProt Related Accession #

P58294

GenBank Nucleotide #

NM_032414.2

NCBI Official Full Name

prokineticin-1

NCBI Official Synonym Full Names

prokineticin 1

NCBI Official Symbol

PROK1

NCBI Official Synonym Symbols

PK1; PRK1; EGVEGF

UniProt Protein Name

Prokineticin-1

UniProt Synonym Protein Names

Endocrine-gland-derived vascular endothelial growth factor; EG-VEGF; Mambakine

Protein Family

Prokineticin

UniProt Gene Name

PROK1

UniProt Synonym Gene Names

EG-VEGF

NCBI Protein Information

prokineticin-1

NCBI Summary for EG-VEGF

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]

UniProt Comments for EG-VEGF

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.

Research Articles on EG-VEGF

1. Results show the biological effects of PROK1-V67I on cell functions are similar to those of wild type, and the common variant of V67I may act as a modifier in the PROK1-PROKR system through down-regulation of PROK1 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EG-VEGF elisa kit

Reference Product	PubMed Publications
PROKR1 elisa kit	>32 publications with EG-VEGF and PROKR1
PROKR2 elisa kit	>21 publications with EG-VEGF and PROKR2
KISS1 elisa kit	>1 publications with EG-VEGF and KISS1
F2R elisa kit	>1 publications with EG-VEGF and F2R

Pathways associated with EG-VEGF elisa kit

Products by Pathway	Pathway Diagram
Circadian Rythm Related Genes Pathway antibodies	Circadian Rythm Related Genes Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with EG-VEGF elisa kit

Disease Name	Pubmed Publications
Premature Birth Antibodies	>3 publications with EG-VEGF and Premature Birth
Disease Models, Animal Antibodies	>2 publications with EG-VEGF and Disease Models, Animal
Hyperplasia Antibodies	>2 publications with EG-VEGF and Hyperplasia
Inflammation Antibodies	>2 publications with EG-VEGF and Inflammation
Nervous System Diseases Antibodies	>1 publications with EG-VEGF and Nervous System Diseases
Liver Diseases Antibodies	>1 publications with EG-VEGF and Liver Diseases

Organs/Tissues associated with EG-VEGF elisa kit

Organ/Tissue Name	Pubmed Publications
Placenta Antibodies	>24 publications with EG-VEGF and Placenta
Uterus Antibodies	>19 publications with EG-VEGF and Uterus
Ovary Antibodies	>13 publications with EG-VEGF and Ovary
Testis Antibodies	>6 publications with EG-VEGF and Testis
Brain Antibodies	>3 publications with EG-VEGF and Brain