Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit for Rat

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of APAF1 in rat tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APAF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APAF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APAF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APAF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of APAF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS450603 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APAF1. The ELISA analytical biochemical technique of the MBS450603 kit is based on APAF1 antibody-APAF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APAF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APAF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).