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CDC34B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4507830

MBS4507830 is an antibody targeting CDC34B. Also known as Rabbit anti-CDC34B Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$250 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDC34B, Polyclonal Antibody
Full Product Name
Rabbit anti-CDC34B Antibody
Product Gene Name
Product Synonym Names
CDC34B; UBC3B; Ubiquitin-conjugating enzyme E2 R2; Ubiquitin carrier protein R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
27,166 Da
OMIM
612506
3D Structure
ModBase 3D Structure for Q712K3
Immunogen
Recombinant full length protein of human CDC34B

Product Categories/Family for anti-CDC34B antibody

ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CDC34B antibody
Western Blot (WB)
Application Notes for anti-CDC34B antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-CDC34B antibody

Western blot analysis of CDC34B expression in Jurkat (A), MCF7 (B), mouse liver (C), mouse kidney (D), rat brain (E), rat lung (F) whole cell lysates.
Rabbit Anti-CDC34B Polyclonal Antibody – Cat# MBS4507830

Immunofluorescence (IF) of anti-CDC34B antibody

Immunofluorescent analysis of CDC34B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-CDC34B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4507830

Other Notes

Small volumes of anti-CDC34B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC34B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 R2
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2R 2
NCBI Official Symbol
UBE2R2
NCBI Official Synonym Symbols
UBC3B; CDC34B; E2-CDC34B
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 R2
UniProt Synonym Protein Names
Ubiquitin carrier protein R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2
UniProt Gene Name
UBE2R2
UniProt Synonym Gene Names
CDC34B; UBC3B
UniProt Entry Name
UB2R2_HUMAN
NCBI Protein Information
ubiquitin-conjugating enzyme E2 R2; ubiquitin carrier protein R2; ubiquitin-conjugating enzyme E2-CDC34B; ubiquitin-conjugating enzyme UBC3B; ubiquitin-protein ligase R2

NCBI Summary for CDC34B

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq, Jul 2008]
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UniProt Comments for CDC34B

UBC3B: an E2 ubiquitin conjugating enzyme similar to UBC3/CDC34. Phosphorylation of UBC3B by CK2 induces its interaction with beta-TrCP, regulating beta-TrCP substrate recognition and enhancing beta-catenin degradation.

Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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