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SMYD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4508289

MBS4508289 is an antibody targeting SMYD3. Also known as Rabbit anti-SMYD3 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$250 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMYD3, Polyclonal Antibody
Full Product Name
Rabbit anti-SMYD3 Antibody
Product Gene Name
anti-SMYD3 antibody
Product Synonym Names
ZMYND1; ZNFN3A1; Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
428
OMIM
608783
3D Structure
ModBase 3D Structure for Q9H7B4
Immunogen
Recombinant full length protein of human SMYD3

Product Categories/Family for anti-SMYD3 antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SMYD3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-SMYD3 antibody
WB: 1:500-1:2000
IF:IC: 1:50-1:200

Western Blot (WB) of anti-SMYD3 antibody

Western blot analysis of SMYD3 expression in Jurkat (A), MCF7 (B), Hela (C), mouse heart (D) whole cell lysates.
Rabbit Anti-SMYD3 Polyclonal Antibody – Cat# MBS4508289

Immunofluorescence (IF) of anti-SMYD3 antibody

Immunofluorescent analysis of SMYD3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-SMYD3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4508289

Other Notes

Small volumes of anti-SMYD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMYD3. It may not necessarily be applicable to this product.

NCBI GI #
212276523
NCBI GeneID
64754
NCBI Accession #
Q9H7B4.4
UniProt Primary Accession #
Q9H7B4
UniProt Secondary Accession #
Q9CWR2
UniProt Related Accession #
Q9H7B4
NCBI Official Full Name
Histone-lysine N-methyltransferase SMYD3
NCBI Official Synonym Full Names
SET and MYND domain containing 3
NCBI Official Symbol
SMYD3
NCBI Official Synonym Symbols
KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
UniProt Protein Name
Histone-lysine N-methyltransferase SMYD3
UniProt Synonym Protein Names
SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
SMYD3
UniProt Synonym Gene Names
ZMYND1; ZNFN3A1
UniProt Entry Name
SMYD3_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase SMYD3; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; SET and MYND domain-containing protein 3; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing)

NCBI Summary for SMYD3

This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for SMYD3

SMYD3: Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: metal ion binding; histone-lysine N-methyltransferase activity

Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; establishment of protein localization; myotube cell development; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation
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Research Articles on SMYD3

1. Mutational analyses revealed that the MYND-domain of SMYD3 and domain III of hepatitis C virus NS5A are required for the interaction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMYD3 antibody

 Reference Product  PubMed Publications
 MET antibody  >2 publications with SMYD3 and MET
 HELZ antibody  >2 publications with SMYD3 and HELZ
 RASSF1 antibody  >1 publications with SMYD3 and RASSF1

Pathways associated with anti-SMYD3 antibody

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 PKMTs Methylate Histone Lysines Pathway antibodies  PKMTs Methylate Histone Lysines Pathway Diagram

Diseases associated with anti-SMYD3 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >14 publications with SMYD3 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >12 publications with SMYD3 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >10 publications with SMYD3 and Liver Neoplasms
 Breast Neoplasms Antibodies  >9 publications with SMYD3 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >4 publications with SMYD3 and Cell Transformation, Neoplastic
 Carcinoma, Squamous Cell Antibodies  >2 publications with SMYD3 and Carcinoma, Squamous Cell
 Inflammation Antibodies  >2 publications with SMYD3 and Inflammation
 Disease Models, Animal Antibodies  >2 publications with SMYD3 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >2 publications with SMYD3 and Neoplasm Metastasis
 Lung Diseases Antibodies  >2 publications with SMYD3 and Lung Diseases

Organs/Tissues associated with anti-SMYD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >15 publications with SMYD3 and Liver
 Muscle Antibodies  >10 publications with SMYD3 and Muscle
 Heart Antibodies  >7 publications with SMYD3 and Heart
 Lung Antibodies  >6 publications with SMYD3 and Lung
 Prostate Antibodies  >3 publications with SMYD3 and Prostate
 Embryonic Tissue Antibodies  >2 publications with SMYD3 and Embryonic Tissue
 Lymph Node Antibodies  >2 publications with SMYD3 and Lymph Node
 Bone Marrow Antibodies  >1 publications with SMYD3 and Bone Marrow
 Vascular Antibodies  >1 publications with SMYD3 and Vascular
 Blood Antibodies  >1 publications with SMYD3 and Blood
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