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Histamine H3 Receptor (H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4509430

MBS4509430 is an antibody targeting H3. Also known as Rabbit anti-Histamine H3 Receptor Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$265 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histamine H3 Receptor, Polyclonal Antibody
Full Product Name
Rabbit anti-Histamine H3 Receptor Antibody
Product Gene Name
anti-H3 antibody
Product Synonym Names
GPCR97; Histamine H3 receptor; H3R; HH3R; G-protein coupled receptor 97
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
48,671 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (60790017..60795323, complement). Location: 20q13.33
OMIM
604525
3D Structure
ModBase 3D Structure for Q9Y5N1
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H3 Receptor. The exact sequence is proprietary.

Product Categories/Family for anti-H3 antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-H3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-H3 antibody
WB: 1:500-1:1000
IHC: 1:100-1:200

Western Blot (WB) of anti-H3 antibody

Western blot analysis of Histamine H3 Receptor expression in HeLa (A), A549 (B), Raw264.7 (C) whole cell lysates.
Rabbit Anti-H3 Polyclonal Antibody – Cat# MBS4509430

Immunohistochemistry (IHC) of anti-H3 antibody

Immunohistochemical analysis of Histamine H3 Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4509430

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
194018562
NCBI GeneID
11255
NCBI Accession #
NP_009163.2
UniProt Primary Accession #
Q9Y5N1
UniProt Secondary Accession #
P58406; Q9QYN8
UniProt Related Accession #
Q9Y5N1
GenBank Nucleotide #
NM_007232.2
NCBI Official Full Name
histamine H3 receptor
NCBI Official Synonym Full Names
histamine receptor H3
NCBI Official Symbol
HRH3
NCBI Official Synonym Symbols
HH3R; GPCR97
UniProt Protein Name
Histamine H3 receptor
UniProt Synonym Protein Names
G-protein coupled receptor 97
Protein Family
Tyrosine phosphatase
UniProt Gene Name
HRH3
UniProt Synonym Gene Names
GPCR97; H3R; HH3R
UniProt Entry Name
HRH3_HUMAN
NCBI Protein Information
histamine H3 receptor; H3R; G protein-coupled receptor 97; G-protein coupled receptor 97

NCBI Summary for H3

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq, Jul 2008]
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UniProt Comments for H3

H3R: The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization. Belongs to the G-protein coupled receptor 1 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane; myelin sheath

Molecular Function: drug binding; histamine receptor activity

Biological Process: negative regulation of glutamate secretion; eating behavior; neurotransmitter secretion; negative regulation of adenylate cyclase activity; negative regulation of gamma-aminobutyric acid secretion; drinking behavior; learning; regulation of norepinephrine secretion; response to organic cyclic substance; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of serotonin secretion; elevation of cytosolic calcium ion concentration; negative regulation of blood pressure; brain development; positive regulation of epithelial cell proliferation
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Research Articles on H3

1. mRNA expression of HRH-3 localized in large pigmented neurons is significantly decreased in the substantia nigra of Parkinson's patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-H3 antibody

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Histamine Receptors Pathway antibodies  Histamine Receptors Pathway Diagram
 Monoamine Transport Pathway antibodies  Monoamine Transport Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with anti-H3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with H3 and Neoplasms
 Neurologic Manifestations Antibodies  >2 publications with H3 and Neurologic Manifestations
 Neurodegenerative Diseases Antibodies  >2 publications with H3 and Neurodegenerative Diseases
 Cardiovascular Diseases Antibodies  >2 publications with H3 and Cardiovascular Diseases
 Inflammation Antibodies  >2 publications with H3 and Inflammation
 Respiratory Hypersensitivity Antibodies  >1 publications with H3 and Respiratory Hypersensitivity
 Edema Antibodies  >1 publications with H3 and Edema

Organs/Tissues associated with anti-H3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with H3 and Brain
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