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Adrenergic Receptor (ALPHA-1D) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4511057

MBS4511057 is an antibody targeting Alpha-1D. Also known as Rabbit anti-Alpha-1D Adrenergic Receptor Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$270 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Alpha-1D Adrenergic Receptor, Polyclonal Antibody
Full Product Name
Rabbit anti-Alpha-1D Adrenergic Receptor Antibody
Product Gene Name
anti-Alpha-1D antibody
Product Synonym Names
ADRA1A; Alpha-1D adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1D adrenoreceptor; Alpha-1D adrenoceptor; Alpha-adrenergic receptor 1a
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
60,463 Da
OMIM
104219
3D Structure
ModBase 3D Structure for P25100
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-1D Adrenergic Receptor. The exact sequence is proprietary.

Product Categories/Family for anti-Alpha-1D antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Alpha-1D antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-Alpha-1D antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF:IC: 1:50-1:100

Western Blot (WB) of anti-Alpha-1D antibody

Western blot analysis of Alpha-1D Adrenergic Receptor expression in MCF7 (A), RAW264.7 (B), H9C2 (C) whole cell lysates.
Rabbit Anti-ALPHA-1D Polyclonal Antibody – Cat# MBS4511057

Immunohistochemistry (IHC) of anti-Alpha-1D antibody

Immunohistochemical analysis of Alpha-1D Adrenergic Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-ALPHA-1D Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4511057

Immunofluorescence (IF) of anti-Alpha-1D antibody

Immunofluorescent analysis of Alpha-1D Adrenergic Receptor staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-ALPHA-1D Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4511057

Other Notes

Small volumes of anti-Alpha-1D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Alpha-1D. It may not necessarily be applicable to this product.

NCBI GI #
4501957
NCBI GeneID
146
NCBI Accession #
NP_000669.1
UniProt Primary Accession #
P25100
UniProt Secondary Accession #
P97714; P23944
UniProt Related Accession #
P25100
GenBank Nucleotide #
NM_000678.3
NCBI Official Full Name
alpha-1D adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor alpha 1D
NCBI Official Symbol
ADRA1D
NCBI Official Synonym Symbols
DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
UniProt Protein Name
Alpha-1D adrenergic receptor
UniProt Synonym Protein Names
Alpha-1A adrenergic receptor; Alpha-1D adrenoreceptor; Alpha-1D adrenoceptor; Alpha-adrenergic receptor 1a
Protein Family
Alpha-1D adrenergic receptor
UniProt Gene Name
ADRA1D
UniProt Synonym Gene Names
ADRA1A; Alpha-1D adrenoceptor
UniProt Entry Name
ADA1D_HUMAN
NCBI Protein Information
alpha-1D adrenergic receptor; alpha-1D adrenoceptor; alpha-1D adrenoreceptor; alpha-1A adrenergic receptor; alpha-1D-adrenergic receptor; alpha-adrenergic receptor 1a; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1D-, receptor; adrenergic, alpha -1D

NCBI Summary for Alpha-1D

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1D-adrenergic receptor. Similar to alpha-1B-adrenergic receptor gene, this gene comprises 2 exons and a single intron that interrupts the coding region. [provided by RefSeq, Jul 2008]
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UniProt Comments for Alpha-1D

ADRA1D: This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1D sub-subfamily.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: alpha1-adrenergic receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; multicellular organismal development; negative regulation of the force of heart contraction during baroreceptor response to increased systemic arterial blood pressure; positive regulation of cell proliferation; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; DNA metabolic process
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Research Articles on Alpha-1D

1. Mean alpha-receptor stain rates in renal pelvis were 2.65 +/- 0.74, 1.35 +/- 0.81 and 2.9 +/- 0.30 for alpha 1A, 1B and 1D, respectively. For calyces, the rates are 2.40 +/- 0.82, 1.50 +/- 0.76 and 2.75 +/- 0.44 for alpha 1A, 1B and 1D, respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Alpha-1D antibody

 Reference Product  PubMed Publications
 ADRBK1 antibody  >2 publications with Alpha-1D and ADRBK1

Pathways associated with anti-Alpha-1D antibody

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenoceptors Pathway antibodies  Adrenoceptors Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with anti-Alpha-1D antibody

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >15 publications with Alpha-1D and Hypertension
 Prostatic Hyperplasia Antibodies  >5 publications with Alpha-1D and Prostatic Hyperplasia
 Hyperplasia Antibodies  >5 publications with Alpha-1D and Hyperplasia
 Heart Failure Antibodies  >4 publications with Alpha-1D and Heart Failure
 Kidney Diseases Antibodies  >4 publications with Alpha-1D and Kidney Diseases
 Nervous System Diseases Antibodies  >4 publications with Alpha-1D and Nervous System Diseases
 Heart Diseases Antibodies  >3 publications with Alpha-1D and Heart Diseases
 Necrosis Antibodies  >3 publications with Alpha-1D and Necrosis
 Edema Antibodies  >2 publications with Alpha-1D and Edema
 Pain Antibodies  >2 publications with Alpha-1D and Pain

Organs/Tissues associated with anti-Alpha-1D antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >19 publications with Alpha-1D and Brain
 Prostate Antibodies  >9 publications with Alpha-1D and Prostate
 Eye Antibodies  >4 publications with Alpha-1D and Eye
 Bone Antibodies  >1 publications with Alpha-1D and Bone
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