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PRDM14 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4511193

MBS4511193 is an antibody targeting PRDM14. Also known as Rabbit anti-PRDM14 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$270 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRDM14, Polyclonal Antibody
Full Product Name
Rabbit anti-PRDM14 Antibody
Product Gene Name
anti-PRDM14 antibody
Product Synonym Names
PR domain zinc finger protein 14; PR domain-containing protein 14
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
64,062 Da
OMIM
AF319458 mRNA
3D Structure
ModBase 3D Structure for Q9GZV8
Immunogen
Recombinant full length protein of human PRDM14

Product Categories/Family for anti-PRDM14 antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PRDM14 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochemistry (IC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-PRDM14 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF:IC: 1:50-1:200
IP: 1:20-1:100
ChIP: 1:20-1:100

Western Blot (WB) of anti-PRDM14 antibody

Western blot analysis of PRDM14 expression in BT474 (A), Jurkat (B), Hela (C) whole cell lysates.
Rabbit Anti-PRDM14 Polyclonal Antibody – Cat# MBS4511193

Immunohistochemistry (IHC) of anti-PRDM14 antibody

Immunohistochemical analysis of PRDM14 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-PRDM14 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4511193

Immunofluorescence (IF) of anti-PRDM14 antibody

Immunofluorescent analysis of PRDM14 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-PRDM14 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4511193

Other Notes

Small volumes of anti-PRDM14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRDM14. It may not necessarily be applicable to this product.

NCBI GI #
13375636
NCBI GeneID
63978
NCBI Accession #
NP_078780.1
UniProt Primary Accession #
Q9GZV8
UniProt Secondary Accession #
E9Q3T6
UniProt Related Accession #
Q9GZV8
GenBank Nucleotide #
NM_024504.3
NCBI Official Full Name
PR domain zinc finger protein 14
NCBI Official Synonym Full Names
PR/SET domain 14
NCBI Official Symbol
PRDM14
NCBI Official Synonym Symbols
PFM11
UniProt Protein Name
PR domain zinc finger protein 14
UniProt Synonym Protein Names
PR domain-containing protein 14
Protein Family
PR domain zinc finger protein
UniProt Gene Name
PRDM14
NCBI Protein Information
PR domain zinc finger protein 14

NCBI Summary for PRDM14

This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]
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UniProt Comments for PRDM14

PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

Protein type: C2H2-type zinc finger protein; Methyltransferase, protein arginine, predicted

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: nucleoplasm

Molecular Function: chromatin DNA binding; metal ion binding; methyltransferase activity; protein binding; RNA binding

Biological Process: cell fate specification; cell morphogenesis; embryo implantation; fertilization; germ cell development; germ-line stem cell maintenance; homeostasis of number of cells within a tissue; inner cell mass cell fate commitment; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of flagellated sperm motility; somatic stem cell maintenance; transcription, DNA-dependent
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Research Articles on PRDM14

1. miR-424-->cdc42-->prdm14 axis as a key molecular signalling cascade that might influence breast cancer progression in diabetic patients through hyperactivation of cancer stem cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PRDM14 antibody

 Reference Product  PubMed Publications
 OCT4 antibody  >6 publications with PRDM14 and OCT4
 NANOG antibody  >5 publications with PRDM14 and NANOG
 SOX2 antibody  >4 publications with PRDM14 and SOX2
 DPPA3 antibody  >3 publications with PRDM14 and DPPA3
 NANOS3 antibody  >1 publications with PRDM14 and NANOS3

Pathways associated with anti-PRDM14 antibody

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Transcriptional Regulation Of Pluripotent Stem Cells Pathway antibodies  Transcriptional Regulation Of Pluripotent Stem Cells Pathway Diagram

Diseases associated with anti-PRDM14 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >7 publications with PRDM14 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >3 publications with PRDM14 and Cell Transformation, Neoplastic
 Respiratory Tract Diseases Antibodies  >3 publications with PRDM14 and Respiratory Tract Diseases
 Immune System Diseases Antibodies  >3 publications with PRDM14 and Immune System Diseases
 Carcinoma in Situ Antibodies  >3 publications with PRDM14 and Carcinoma in Situ
 Precursor Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >3 publications with PRDM14 and Precursor Cell Lymphoblastic Leukemia-Lymphoma
 Neoplasm Metastasis Antibodies  >2 publications with PRDM14 and Neoplasm Metastasis
 Female Urogenital Diseases Antibodies  >2 publications with PRDM14 and Female Urogenital Diseases
 Endocrine Gland Neoplasms Antibodies  >1 publications with PRDM14 and Endocrine Gland Neoplasms
 Infertility, Male Antibodies  >1 publications with PRDM14 and Infertility, Male

Organs/Tissues associated with anti-PRDM14 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with PRDM14 and Testis
 Embryonic Tissue Antibodies  >1 publications with PRDM14 and Embryonic Tissue
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