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SNRPA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4511217

MBS4511217 is an antibody targeting SNRPA. Also known as Rabbit anti-SNRPA Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$270 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SNRPA, Polyclonal Antibody
Full Product Name
Rabbit anti-SNRPA Antibody
Product Gene Name
Product Synonym Names
U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1-A; U1A
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
31,280 Da
OMIM
182285
3D Structure
ModBase 3D Structure for P09012
Immunogen
Recombinant full length protein of human SNRPA

Product Categories/Family for anti-SNRPA antibody

ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SNRPA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-SNRPA antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF:IC: 1:50-1:200

Western Blot (WB) of anti-SNRPA antibody

Western blot analysis of SNRPA expression in A431 (A), MCF7 (B), mouse testis (C), rat brain (D) whole cell lysates.
Rabbit Anti-SNRPA Polyclonal Antibody – Cat# MBS4511217

Immunohistochemistry (IHC) of anti-SNRPA antibody

Immunohistochemical analysis of SNRPA staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-SNRPA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4511217

Immunofluorescence (IF) of anti-SNRPA antibody

Immunofluorescent analysis of SNRPA staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-SNRPA Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4511217

Other Notes

Small volumes of anti-SNRPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SNRPA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
U1 small nuclear ribonucleoprotein A
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein polypeptide A
NCBI Official Symbol
SNRPA
NCBI Official Synonym Symbols
U1A; Mud1; U1-A
UniProt Protein Name
U1 small nuclear ribonucleoprotein A
UniProt Gene Name
SNRPA
UniProt Synonym Gene Names
U1 snRNP A; U1-A; U1A
UniProt Entry Name
SNRPA_HUMAN
NCBI Protein Information
U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1 snRNP-specific protein A; U1 small nuclear RNP-specific A

NCBI Summary for SNRPA

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]
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UniProt Comments for SNRPA

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SNRPA antibody

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