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14-3-3 Zeta (14-3-3zeta) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4512628

MBS4512628 is an antibody targeting 14-3-3zeta. Also known as Rabbit anti-14-3-3 zeta Antibody. Reactive in Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep. Host: Rabbit. More details

$275 / 0.05 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
14-3-3 zeta (14-3-3zeta), Polyclonal Antibody
Full Product Name
Rabbit anti-14-3-3 zeta Antibody
Product Gene Name
anti-14-3-3zeta antibody
Product Synonym Names
14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
27,745 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (101930804..101965623, complement). Location: 8q23.1
OMIM
601288
3D Structure
ModBase 3D Structure for P63104
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 14-3-3 zeta. The exact sequence is proprietary.

Product Categories/Family for anti-14-3-3zeta antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-14-3-3zeta antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-14-3-3zeta antibody
WB: 1:500-1:1000
IHC: 1:100-1:200
IF:IC: 1:100-1:500

Western Blot (WB) of anti-14-3-3zeta antibody

Western blot analysis of 14-3-3 zeta expression in MCF7 (A), HeLa (B), SP2/0 (C), H9C2 (D) whole cell lysates.
Rabbit Anti-14-3-3zeta Polyclonal Antibody – Cat# MBS4512628

Immunohistochemistry (IHC) of anti-14-3-3zeta antibody

Immunohistochemical analysis of 14-3-3 zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-14-3-3zeta Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4512628

Immunofluorescence (IF) of anti-14-3-3zeta antibody

Immunofluorescent analysis of 14-3-3 zeta staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-14-3-3zeta Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4512628

Other Notes

Small volumes of anti-14-3-3zeta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for 14-3-3zeta. It may not necessarily be applicable to this product.

NCBI GI #
208973238
NCBI GeneID
7534
NCBI Accession #
NP_001129171.1
UniProt Primary Accession #
P63104
UniProt Secondary Accession #
P63101; P63102
UniProt Related Accession #
P63104
GenBank Nucleotide #
NM_001135699.1
NCBI Official Full Name
14-3-3 protein zeta/delta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
NCBI Official Symbol
YWHAZ
NCBI Official Synonym Symbols
YWHAD; KCIP-1; 14-3-3-zeta
UniProt Protein Name
14-3-3 protein zeta/delta
UniProt Synonym Protein Names
Protein kinase C inhibitor protein 1
Protein Family
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
UniProt Gene Name
YWHAZ
UniProt Synonym Gene Names
KCIP-1
UniProt Entry Name
1433Z_HUMAN
NCBI Protein Information
14-3-3 protein zeta/delta; 14-3-3 zeta; 14-3-3 delta; phospholipase A2; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; 14-3-3 protein/cytosolic phospholipase A2; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta

NCBI Summary for 14-3-3zeta

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]
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UniProt Comments for 14-3-3zeta

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; protein complex; mast cell granule; cytoplasmic vesicle membrane; focal adhesion; mitochondrion; postsynaptic density; leading edge; cytosol; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus

Molecular Function: identical protein binding; protein domain specific binding; protein binding; protein complex binding; protein kinase binding; transcription factor binding

Biological Process: response to drug; platelet activation; histamine secretion by mast cell; establishment of Golgi localization; apoptosis; gene expression; signal transduction; blood coagulation; protein targeting to mitochondrion; negative regulation of apoptosis
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Research Articles on 14-3-3zeta

1. Overexpression of YWHAZ relates to tumor cell proliferation and malignant outcome of gastric carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-14-3-3zeta antibody

 Reference Product  PubMed Publications
 TUBB antibody  >4 publications with 14-3-3zeta and TUBB
 YWHAE antibody  >3 publications with 14-3-3zeta and YWHAE
 BCR antibody  >2 publications with 14-3-3zeta and BCR
 VIM antibody  >2 publications with 14-3-3zeta and VIM
 LIMK1 antibody  >1 publications with 14-3-3zeta and LIMK1
 IRS1 antibody  >1 publications with 14-3-3zeta and IRS1
 FOXO3 antibody  >1 publications with 14-3-3zeta and FOXO3
 EGFR antibody  >1 publications with 14-3-3zeta and EGFR
 CFL1 antibody  >1 publications with 14-3-3zeta and CFL1
 ABL1 antibody  >1 publications with 14-3-3zeta and ABL1

Pathways associated with anti-14-3-3zeta antibody

 Products by Pathway  Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram

Diseases associated with anti-14-3-3zeta antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >28 publications with 14-3-3zeta and Neoplasms
 Carcinoma Antibodies  >12 publications with 14-3-3zeta and Carcinoma
 Brain Diseases Antibodies  >10 publications with 14-3-3zeta and Brain Diseases
 Nervous System Diseases Antibodies  >9 publications with 14-3-3zeta and Nervous System Diseases
 Breast Neoplasms Antibodies  >7 publications with 14-3-3zeta and Breast Neoplasms
 Skin Diseases Antibodies  >7 publications with 14-3-3zeta and Skin Diseases
 Cardiovascular Diseases Antibodies  >6 publications with 14-3-3zeta and Cardiovascular Diseases
 Inflammation Antibodies  >5 publications with 14-3-3zeta and Inflammation
 Heart Diseases Antibodies  >4 publications with 14-3-3zeta and Heart Diseases
 Drug Toxicity Antibodies  >3 publications with 14-3-3zeta and Drug Toxicity

Organs/Tissues associated with anti-14-3-3zeta antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >21 publications with 14-3-3zeta and Brain
 Blood Antibodies  >18 publications with 14-3-3zeta and Blood
 Liver Antibodies  >13 publications with 14-3-3zeta and Liver
 Muscle Antibodies  >10 publications with 14-3-3zeta and Muscle
 Heart Antibodies  >8 publications with 14-3-3zeta and Heart
 Lung Antibodies  >6 publications with 14-3-3zeta and Lung
 Kidney Antibodies  >5 publications with 14-3-3zeta and Kidney
 Prostate Antibodies  >4 publications with 14-3-3zeta and Prostate
 Stomach Antibodies  >4 publications with 14-3-3zeta and Stomach
 Embryonic Tissue Antibodies  >4 publications with 14-3-3zeta and Embryonic Tissue
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