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Cytokeratin 20 (KRT20) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4512681

MBS4512681 is an antibody targeting KRT20. Also known as Rabbit anti-Cytokeratin 20 Antibody. Reactive in Human, Mouse, Rat, Bovine, Pig. Host: Rabbit. More details

$275 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 20 (KRT20), Polyclonal Antibody
Full Product Name
Rabbit anti-Cytokeratin 20 Antibody
Product Gene Name
anti-KRT20 antibody
Product Synonym Names
Keratin, type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
48,487 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (39032141..39041495, complement). Location: 17q21.2
OMIM
608218
3D Structure
ModBase 3D Structure for P35900
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 20. The exact sequence is proprietary.

Product Categories/Family for anti-KRT20 antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KRT20 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-KRT20 antibody
WB: 1:500-1:1000
IHC: 1:100-1:200
IF:IC: 1:100-1:500

Western Blot (WB) of anti-KRT20 antibody

Western blot analysis of Cytokeratin 20 expression in A549 (A), NS-1 (B), mouse kidney (C), rat kidney (D) whole cell lysates.
Rabbit Anti-KRT20 Polyclonal Antibody – Cat# MBS4512681

Immunohistochemistry (IHC) of anti-KRT20 antibody

Immunohistochemical analysis of Cytokeratin 20 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-KRT20 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4512681

Immunofluorescence (IF) of anti-KRT20 antibody

Immunofluorescent analysis of Cytokeratin 20 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-KRT20 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4512681

Other Notes

Small volumes of anti-KRT20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT20. It may not necessarily be applicable to this product.

NCBI GI #
27894337
NCBI GeneID
54474
NCBI Accession #
NP_061883.1
UniProt Primary Accession #
P35900
UniProt Secondary Accession #
Q9D312; P25030
UniProt Related Accession #
P35900
GenBank Nucleotide #
NM_019010.2
NCBI Official Full Name
keratin, type I cytoskeletal 20
NCBI Official Synonym Full Names
keratin 20
NCBI Official Symbol
KRT20
NCBI Official Synonym Symbols
K20; CD20; CK20; CK-20; KRT21
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Synonym Protein Names
Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT
Protein Family
Keratin
UniProt Gene Name
KRT20
UniProt Synonym Gene Names
CK-20; K20
UniProt Entry Name
K1C20_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 20; keratin-20; protein IT; cytokeratin 20; cytokeratin-20

NCBI Summary for KRT20

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT20

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization
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Research Articles on KRT20

1. Overexpression of CK20 is associated with early-onset colorectal cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT20 antibody

 Reference Product  PubMed Publications
 VIM antibody  >2 publications with KRT20 and VIM

Diseases associated with anti-KRT20 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >471 publications with KRT20 and Neoplasms
 Carcinoma Antibodies  >371 publications with KRT20 and Carcinoma
 Adenocarcinoma Antibodies  >248 publications with KRT20 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >148 publications with KRT20 and Neoplasm Metastasis
 Urinary Bladder Neoplasms Antibodies  >78 publications with KRT20 and Urinary Bladder Neoplasms
 Colonic Neoplasms Antibodies  >72 publications with KRT20 and Colonic Neoplasms
 Lung Neoplasms Antibodies  >67 publications with KRT20 and Lung Neoplasms
 Skin Neoplasms Antibodies  >55 publications with KRT20 and Skin Neoplasms
 Ovarian Neoplasms Antibodies  >54 publications with KRT20 and Ovarian Neoplasms
 Stomach Neoplasms Antibodies  >46 publications with KRT20 and Stomach Neoplasms

Organs/Tissues associated with anti-KRT20 antibody

 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >73 publications with KRT20 and Stomach
 Intestine Antibodies  >42 publications with KRT20 and Intestine
 Kidney Antibodies  >21 publications with KRT20 and Kidney
 Muscle Antibodies  >10 publications with KRT20 and Muscle
 Brain Antibodies  >8 publications with KRT20 and Brain
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