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TRAP100 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4512748

MBS4512748 is an antibody targeting TRAP100. Also known as Rabbit anti-TRAP100 Antibody. Reactive in Human, Rat, Bovine, Chicken, Pig. Host: Rabbit. More details

$275 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRAP100, Polyclonal Antibody
Full Product Name
Rabbit anti-TRAP100 Antibody
Product Gene Name
anti-TRAP100 antibody
Product Synonym Names
ARC100; CRSP4; DRIP100; KIAA0130; THRAP4; TRAP100; Mediator of RNA polymerase II transcription subunit 24; Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; Mediator complex subunit 24; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; hTRAP100; Vitamin D3 receptor-interacting protein complex 100 kDa component; DRIP100
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Bovine, Chicken, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
110,305 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (38175350..38210889, complement). Location: 17q21.1
OMIM
607000
3D Structure
ModBase 3D Structure for O75448
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TRAP100. The exact sequence is proprietary.

Product Categories/Family for anti-TRAP100 antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TRAP100 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-TRAP100 antibody
WB: 1:500-1:1000
IHC: 1:100-1:200
IF:IC: 1:100-1:500

Western Blot (WB) of anti-TRAP100 antibody

Western blot analysis of TRAP100 expression in HepG2 (A), HEK293T (B), Hela (C) whole cell lysates.
Rabbit Anti-TRAP100 Polyclonal Antibody – Cat# MBS4512748

Immunohistochemistry (IHC) of anti-TRAP100 antibody

Immunohistochemical analysis of TRAP100 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-TRAP100 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4512748

Immunofluorescence (IF) of anti-TRAP100 antibody

Immunofluorescent analysis of TRAP100 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-TRAP100 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4512748

Other Notes

Small volumes of anti-TRAP100 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRAP100. It may not necessarily be applicable to this product.

NCBI GI #
119220575
NCBI GeneID
9862
NCBI Accession #
NP_001072986.1
UniProt Primary Accession #
O75448
UniProt Secondary Accession #
Rat:Q4V8B3
UniProt Related Accession #
O75448
GenBank Nucleotide #
NM_001079518.1
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 24 isoform 2
NCBI Official Synonym Full Names
mediator complex subunit 24
NCBI Official Symbol
MED24
NCBI Official Synonym Symbols
CRSP4; ARC100; THRAP4; CRSP100; DRIP100; TRAP100
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 24
UniProt Synonym Protein Names
Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; Mediator complex subunit 24; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; hTRAP100; Vitamin D3 receptor-interacting protein complex 100 kDa component
Protein Family
Mediator of RNA polymerase II transcription
UniProt Gene Name
MED24
UniProt Synonym Gene Names
ARC100; CRSP4; DRIP100; KIAA0130; THRAP4; TRAP100; ARC100; CRSP complex subunit 4; Trap100; hTRAP100; DRIP100
UniProt Entry Name
MED24_HUMAN
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 24; CRSP complex subunit 4; thyroid hormone receptor associated protein 4; thyroid hormone receptor-associated protein 4; activator-recruited cofactor 100 kDa component; cofactor required for Sp1 transcr

NCBI Summary for TRAP100

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for TRAP100

Trap100: a transcriptional coactivator protein of the thyroid hormone receptor-associated protein (TRAP) family. Forms a complex with the thyroid hormone receptor (TR) and markedly enhance TR-mediated transcription in vitro. Interacts and co-precipitates with Trap220 but does not directly bind nuclear hormone receptors.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: nucleoplasm; Srb-mediator complex; nucleus

Molecular Function: protein binding; histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; thyroid hormone receptor binding; receptor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; protein heterooligomerization; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; stem cell maintenance; gene expression; histone acetylation
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Research Articles on TRAP100

1. Study identified significantly white blood cell count (WBC) level associated SNPs of three separate genes GSDMA, MED24, and PSMD3 in European continent (EA) subjects.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRAP100 antibody

 Reference Product  PubMed Publications
 PPARG antibody  >1 publications with TRAP100 and PPARG

Pathways associated with anti-TRAP100 antibody

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with anti-TRAP100 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >3 publications with TRAP100 and Breast Neoplasms
 Death Antibodies  >3 publications with TRAP100 and Death
 Immune System Diseases Antibodies  >1 publications with TRAP100 and Immune System Diseases
 Neoplasm Metastasis Antibodies  >1 publications with TRAP100 and Neoplasm Metastasis
 Prostatic Diseases Antibodies  >1 publications with TRAP100 and Prostatic Diseases
 Mammary Neoplasms, Animal Antibodies  >1 publications with TRAP100 and Mammary Neoplasms, Animal

Organs/Tissues associated with anti-TRAP100 antibody

 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >3 publications with TRAP100 and Thyroid
 Salivary Gland Antibodies  >2 publications with TRAP100 and Salivary Gland
 Mouth Antibodies  >2 publications with TRAP100 and Mouth
 Blood Antibodies  >1 publications with TRAP100 and Blood
 Eye Antibodies  >1 publications with TRAP100 and Eye
 Kidney Antibodies  >1 publications with TRAP100 and Kidney
 Prostate Antibodies  >1 publications with TRAP100 and Prostate
 Liver Antibodies  >1 publications with TRAP100 and Liver
 Mammary Gland Antibodies  >1 publications with TRAP100 and Mammary Gland
 Embryonic Tissue Antibodies  >1 publications with TRAP100 and Embryonic Tissue
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