Big Dynorphin (DYN) ELISA Kit for Mouse

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Dyn in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dyn has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dyn and unlabeled Dyn (Standards or samples) with the pre-coated antibody specific to Dyn. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dyn in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dyn in the sample.

Small volumes of Dyn elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS451453 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Big Dynorphin (Dyn) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Dyn. The ELISA analytical biochemical technique of the MBS451453 kit is based on Dyn antibody-Dyn antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Dyn antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Dyn. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).