Nucleophosmin (NPM) ELISA Kit for Human

Name: Human NPM ELISA Kit; Nucleophosmin; MyBioSource
Brand: MyBioSource
SKU: MBS451487
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS451487

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NPM in human tissue homogenates, cell lysates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Nucleophosmin (NPM), ELISA Kit

Full Product Name

Human Nucleophosmin (NPM) ELISA Kit

Product Gene Name

NPM elisa kit

Product Synonym Names

B23; NPM1; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family, Member 1; Nucleolar protein NO38

Species Reactivity

Human

Assay Type

Sandwich

Samples

Tissue homogenates, cell lysates and other biological fluids.

Detection Range

0.312-20ng/mL

Sensitivity

0.128ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of NPM. No significant cross-reactivity or interference between NPM and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

28,400 Da

OMIM

M23613 mRNA

3D Structure

ModBase 3D Structure for P06748

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of NPM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPM purchase

MBS451487 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nucleophosmin (NPM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPM. The ELISA analytical biochemical technique of the MBS451487 kit is based on NPM antibody-NPM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NPM. It may not necessarily be applicable to this product.

NCBI GI #

83641870

NCBI GeneID

4869

NCBI Accession #

NP_001032827.1

UniProt Primary Accession #

P06748

UniProt Secondary Accession #

P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6

UniProt Related Accession #

P06748

GenBank Nucleotide #

NM_001037738.2

NCBI Official Full Name

nucleophosmin isoform 3

NCBI Official Synonym Full Names

nucleophosmin

NCBI Official Symbol

NPM1

NCBI Official Synonym Symbols

B23; NPM

UniProt Protein Name

Nucleophosmin

UniProt Synonym Protein Names

Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

Protein Family

Nucleophosmin

UniProt Gene Name

NPM1

UniProt Synonym Gene Names

NPM; NPM

NCBI Protein Information

nucleophosmin

NCBI Summary for NPM

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

UniProt Comments for NPM

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).

Research Articles on NPM

1. Molecular subtypes of NPM1 mutations have different clinical profiles, specific patterns of accompanying molecular mutations and varying outcomes in intermediate risk acute myeloid leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NPM elisa kit

Reference Product	PubMed Publications
TP53 elisa kit	>50 publications with NPM and TP53
MYC elisa kit	>21 publications with NPM and MYC
MDM2 elisa kit	>9 publications with NPM and MDM2
NCL elisa kit	>7 publications with NPM and NCL
CDKN2A elisa kit	>5 publications with NPM and CDKN2A
CDK2 elisa kit	>1 publications with NPM and CDK2

Pathways associated with NPM elisa kit

Products by Pathway	Pathway Diagram
Aurora B Signaling Pathway antibodies	Aurora B Signaling Pathway Diagram
BARD1 Signaling Events Pathway antibodies	BARD1 Signaling Events Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Chromosome Maintenance Pathway antibodies	Chromosome Maintenance Pathway Diagram
Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies	Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram

Diseases associated with NPM elisa kit

Disease Name	Pubmed Publications
Leukemia, Myeloid, Acute Antibodies	>844 publications with NPM and Leukemia, Myeloid, Acute
Leukemia, Promyelocytic, Acute Antibodies	>40 publications with NPM and Leukemia, Promyelocytic, Acute
Cell Transformation, Neoplastic Antibodies	>38 publications with NPM and Cell Transformation, Neoplastic
Carcinoma Antibodies	>32 publications with NPM and Carcinoma
Anemia Antibodies	>18 publications with NPM and Anemia
Adenocarcinoma Antibodies	>17 publications with NPM and Adenocarcinoma
Breast Neoplasms Antibodies	>11 publications with NPM and Breast Neoplasms
Neoplasms, Experimental Antibodies	>7 publications with NPM and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>6 publications with NPM and Carcinoma, Hepatocellular
Neoplasm Metastasis Antibodies	>6 publications with NPM and Neoplasm Metastasis

Organs/Tissues associated with NPM elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>302 publications with NPM and Blood
Bone Antibodies	>205 publications with NPM and Bone
Bone Marrow Antibodies	>202 publications with NPM and Bone Marrow
Lung Antibodies	>16 publications with NPM and Lung
Brain Antibodies	>12 publications with NPM and Brain
Skin Antibodies	>9 publications with NPM and Skin
Prostate Antibodies	>7 publications with NPM and Prostate
Heart Antibodies	>7 publications with NPM and Heart
Muscle Antibodies	>5 publications with NPM and Muscle
Liver Antibodies	>5 publications with NPM and Liver