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Histone Deacetylase 1 (HDAC1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS451600

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HDAC1 in human tissue homogenates, cell lysates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone Deacetylase 1 (HDAC1), ELISA Kit
Full Product Name
Human Histone Deacetylase 1 (HDAC1) ELISA Kit
Product Gene Name
HDAC1 elisa kit
Product Synonym Names
HDAC1; GON10; HD1; RPD3; RPD3L1
Species Reactivity
Human
Assay Type
Sandwich
Samples
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.061ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of HDAC1. No significant cross-reactivity or interference between HDAC1 and analogues was observed.
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HDAC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HDAC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
55,103 Da
OMIM
U50079 mRNA
3D Structure
ModBase 3D Structure for Q13547
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of HDAC1 elisa kit

Human HDAC1 ELISA Kit – Cat# MBS451600

Other Notes

Small volumes of HDAC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HDAC1 purchase

MBS451600 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histone Deacetylase 1 (HDAC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HDAC1. The ELISA analytical biochemical technique of the MBS451600 kit is based on HDAC1 antibody-HDAC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HDAC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HDAC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HDAC1. It may not necessarily be applicable to this product.

NCBI GI #
13128860
NCBI GeneID
3065
NCBI Accession #
NP_004955.2
UniProt Primary Accession #
Q13547
UniProt Secondary Accession #
Q92534
UniProt Related Accession #
Q13547
GenBank Nucleotide #
NM_004964.2
NCBI Official Full Name
histone deacetylase 1
NCBI Official Synonym Full Names
histone deacetylase 1
NCBI Official Symbol
HDAC1
NCBI Official Synonym Symbols
HD1; RPD3; GON-10; RPD3L1
UniProt Protein Name
Histone deacetylase 1
UniProt Gene Name
HDAC1
UniProt Synonym Gene Names
RPD3L1; HD1
NCBI Protein Information
histone deacetylase 1

NCBI Summary for HDAC1

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
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UniProt Comments for HDAC1

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.
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Research Articles on HDAC1

1. High HDAC1 expression is associated with osteosarcoma progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HDAC1 elisa kit

 Reference Product  PubMed Publications
 DNMT1 elisa kit  >139 publications with HDAC1 and DNMT1
 SIN3A elisa kit  >99 publications with HDAC1 and SIN3A
 KDM1A elisa kit  >24 publications with HDAC1 and KDM1A
 MTA2 elisa kit  >18 publications with HDAC1 and MTA2
 RBBP4 elisa kit  >17 publications with HDAC1 and RBBP4
 RCOR1 elisa kit  >16 publications with HDAC1 and RCOR1
 CHD4 elisa kit  >16 publications with HDAC1 and CHD4
 SIN3B elisa kit  >13 publications with HDAC1 and SIN3B
 RBBP7 elisa kit  >9 publications with HDAC1 and RBBP7
 CHD3 elisa kit  >5 publications with HDAC1 and CHD3

Pathways associated with HDAC1 elisa kit

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 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram

Diseases associated with HDAC1 elisa kit

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >118 publications with HDAC1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >112 publications with HDAC1 and Adenocarcinoma
 Inflammation Antibodies  >100 publications with HDAC1 and Inflammation
 Nervous System Diseases Antibodies  >86 publications with HDAC1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >81 publications with HDAC1 and Disease Models, Animal
 Necrosis Antibodies  >70 publications with HDAC1 and Necrosis
 Liver Diseases Antibodies  >68 publications with HDAC1 and Liver Diseases
 Lung Neoplasms Antibodies  >60 publications with HDAC1 and Lung Neoplasms
 Brain Diseases Antibodies  >57 publications with HDAC1 and Brain Diseases
 Cardiovascular Diseases Antibodies  >52 publications with HDAC1 and Cardiovascular Diseases

Organs/Tissues associated with HDAC1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >187 publications with HDAC1 and Brain
 Blood Antibodies  >168 publications with HDAC1 and Blood
 Lung Antibodies  >141 publications with HDAC1 and Lung
 Liver Antibodies  >132 publications with HDAC1 and Liver
 Muscle Antibodies  >96 publications with HDAC1 and Muscle
 Prostate Antibodies  >79 publications with HDAC1 and Prostate
 Bone Antibodies  >75 publications with HDAC1 and Bone
 Heart Antibodies  >66 publications with HDAC1 and Heart
 Kidney Antibodies  >57 publications with HDAC1 and Kidney
 Vascular Antibodies  >56 publications with HDAC1 and Vascular
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