Protein Tyrosine Phosphatase Like Protein A (PTPLA) ELISA Kit for Human

Name: Human PTPLA ELISA Kit; Protein Tyrosine Phosphatase Like Protein A; MyBioSource
Brand: MyBioSource
SKU: MBS451839
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS451839

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTPLA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Protein Tyrosine Phosphatase Like Protein A (PTPLA), ELISA Kit

Full Product Name

Human Protein Tyrosine Phosphatase Like Protein A (PTPLA) ELISA Kit

Product Gene Name

PTPLA elisa kit

Product Synonym Names

CAP; HACD1; Proline Instead Of Catalytic Arginine Member a; 3-hydroxyacyl-CoA dehydratase 1; Cementum Attachment Protein; Very-long-chain 3R-3-hydroxyacyl-CoA dehydratase 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human tissue homogenates, cell lysates or other biological fluids

Detection Range

0.781-50ng/mL

Sensitivity

< 0.25ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of PTPLA. No significant cross-reactivity or interference between PTPLA and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PTPLA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PTPLA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

14,920 Da

3D Structure

ModBase 3D Structure for B0YJ81

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PTPLA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTPLA purchase

MBS451839 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Tyrosine Phosphatase Like Protein A (PTPLA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTPLA. The ELISA analytical biochemical technique of the MBS451839 kit is based on PTPLA antibody-PTPLA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTPLA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTPLA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PTPLA. It may not necessarily be applicable to this product.

NCBI GI #

82659105

NCBI GeneID

9200

NCBI Accession #

NP_055056.3

UniProt Primary Accession #

B0YJ81

UniProt Secondary Accession #

Q6JIC5; Q96FW7; Q9HB93; Q9UHX2; B0YJ80

UniProt Related Accession #

B0YJ81

GenBank Nucleotide #

NM_014241.3

NCBI Official Full Name

very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1

NCBI Official Synonym Full Names

3-hydroxyacyl-CoA dehydratase 1

NCBI Official Symbol

HACD1

NCBI Official Synonym Symbols

CAP; PTPLA

UniProt Protein Name

Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1

UniProt Synonym Protein Names

3-hydroxyacyl-CoA dehydratase 1

UniProt Gene Name

HACD1

UniProt Synonym Gene Names

CAP

NCBI Protein Information

very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1

NCBI Summary for PTPLA

The protein encoded by this gene contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation. [provided by RefSeq, Jul 2008]

UniProt Comments for PTPLA

Isoform 1: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.

Research Articles on PTPLA

1. These data indicate that HACD1 is necessary for muscle function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PTPLA elisa kit

Reference Product	PubMed Publications
PTPLA elisa kit	>4 publications with PTPLA and PTPLA

Pathways associated with PTPLA elisa kit

Products by Pathway	Pathway Diagram
Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies	Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies	Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
Fatty Acyl-CoA Biosynthesis Pathway antibodies	Fatty Acyl-CoA Biosynthesis Pathway Diagram
Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies	Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies	Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
Fatty Acid Elongation Pathway antibodies	Fatty Acid Elongation Pathway Diagram
Fatty Acid Elongation Pathway antibodies	Fatty Acid Elongation Pathway Diagram
Fatty Acid Metabolism Pathway antibodies	Fatty Acid Metabolism Pathway Diagram
Fatty Acid Metabolism Pathway antibodies	Fatty Acid Metabolism Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram

Diseases associated with PTPLA elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>1 publications with PTPLA and Heart Diseases
Nervous System Diseases Antibodies	>1 publications with PTPLA and Nervous System Diseases

Organs/Tissues associated with PTPLA elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>3 publications with PTPLA and Muscle
Heart Antibodies	>1 publications with PTPLA and Heart