Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit for Human

Name: Human ENPP1 ELISA Kit; Ectonucleotide Pyrophosphatase/Phosphodiesterase 1; MyBioSource
Brand: MyBioSource
SKU: MBS451862
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS451862

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ENPP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), ELISA Kit

Full Product Name

Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit

Product Gene Name

ENPP1 elisa kit

Product Synonym Names

M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1; Membrane component chromosome 6 surface marker 1; Alkaline phosphodiesterase I; Plasma-cell membrane glycoprotein PC-1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma, tissue homogenates or other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

< 0.062ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of ENPP1. No significant cross-reactivity or interference between ENPP1 and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ENPP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ENPP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

104,924 Da

OMIM

M57736 mRNA

3D Structure

ModBase 3D Structure for P22413

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of ENPP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENPP1 purchase

MBS451862 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENPP1. The ELISA analytical biochemical technique of the MBS451862 kit is based on ENPP1 antibody-ENPP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENPP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENPP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ENPP1. It may not necessarily be applicable to this product.

NCBI GI #

170650661

NCBI GeneID

5167

NCBI Accession #

NP_006199.2

UniProt Primary Accession #

P22413

UniProt Secondary Accession #

Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3

UniProt Related Accession #

P22413

GenBank Nucleotide #

NM_006208.2

NCBI Official Full Name

ectonucleotide pyrophosphatase/phosphodiesterase family member 1

NCBI Official Synonym Full Names

ectonucleotide pyrophosphatase/phosphodiesterase 1

NCBI Official Symbol

ENPP1

NCBI Official Synonym Symbols

M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1

UniProt Protein Name

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

UniProt Synonym Protein Names

Nucleotide diphosphataseCurated

Protein Family

Ectonucleotide pyrophosphatase/phosphodiesterase family

UniProt Gene Name

ENPP1

UniProt Synonym Gene Names

M6S1; NPPS; PC1; PDNP1; NPPase

NCBI Protein Information

ectonucleotide pyrophosphatase/phosphodiesterase family member 1

NCBI Summary for ENPP1

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

UniProt Comments for ENPP1

By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity and function.

Research Articles on ENPP1

1. In this exploratory analysis, IRS1, ENNP1 and TRIB3, known to be associated with type 2 diabetes and harboring genes playing a prominent role in mediating insulin signaling, may modulate a number of cardiometabolic phenotypes in patients of Italian ancestry with newly-diagnosed type 2 diabetes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ENPP1 elisa kit

Reference Product	PubMed Publications
FGF23 elisa kit	>20 publications with ENPP1 and FGF23
ALPL elisa kit	>12 publications with ENPP1 and ALPL
NT5E elisa kit	>6 publications with ENPP1 and NT5E
IBSP elisa kit	>2 publications with ENPP1 and IBSP
ACP5 elisa kit	>1 publications with ENPP1 and ACP5

Pathways associated with ENPP1 elisa kit

Products by Pathway	Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Vitamins And Cofactors Pathway antibodies	Metabolism Of Vitamins And Cofactors Pathway Diagram
Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies	Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Pantothenate And CoA Biosynthesis Pathway antibodies	Pantothenate And CoA Biosynthesis Pathway Diagram
Pantothenate And CoA Biosynthesis Pathway antibodies	Pantothenate And CoA Biosynthesis Pathway Diagram

Diseases associated with ENPP1 elisa kit

Disease Name	Pubmed Publications
Diabetes Mellitus, Type 2 Antibodies	>68 publications with ENPP1 and Diabetes Mellitus, Type 2
Obesity Antibodies	>62 publications with ENPP1 and Obesity
Arterial calcification of infancy Antibodies	>42 publications with ENPP1 and Arterial calcification of infancy
Kidney Diseases Antibodies	>30 publications with ENPP1 and Kidney Diseases
Disease Models, Animal Antibodies	>22 publications with ENPP1 and Disease Models, Animal
Inflammation Antibodies	>12 publications with ENPP1 and Inflammation
Hypertension Antibodies	>12 publications with ENPP1 and Hypertension
Liver Diseases Antibodies	>10 publications with ENPP1 and Liver Diseases
Hypophosphatemic Rickets, Autosomal Recessive, 2 Antibodies	>6 publications with ENPP1 and Hypophosphatemic Rickets, Autosomal Recessive, 2
Fatty Liver Antibodies	>5 publications with ENPP1 and Fatty Liver

Organs/Tissues associated with ENPP1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>75 publications with ENPP1 and Bone
Blood Antibodies	>64 publications with ENPP1 and Blood
Connective Tissue Antibodies	>32 publications with ENPP1 and Connective Tissue
Heart Antibodies	>29 publications with ENPP1 and Heart
Skin Antibodies	>24 publications with ENPP1 and Skin
Kidney Antibodies	>23 publications with ENPP1 and Kidney
Muscle Antibodies	>23 publications with ENPP1 and Muscle
Liver Antibodies	>19 publications with ENPP1 and Liver
Embryonic Tissue Antibodies	>7 publications with ENPP1 and Embryonic Tissue
Pancreas Antibodies	>6 publications with ENPP1 and Pancreas